ArticleScientific reports2022
Amplification and sequencing of entire tick mitochondrial genomes for a phylogenomic analysis.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 56 citations in OpenAlex.
- Revisiting Ornithodoros (Pavlovskyella) nicollei (Ixodida: Argasidae): new records and first molecular data from Mexico.Experimental & applied acarology · 2026Article
- Northernmost record of Ornithodoros (Alectorobius) puertoricensis (Acari: Argasidae) in Coahuila, Mexico.Experimental & applied acarology · 2026Article
- First description of the complete mitochondrial genomes of the species Amblyomma humerale and Amblyomma geayi (Acari: Ixodidae), Amazon, Pará, Brazil.Experimental & applied acarology · 2026Article
- Quantitative assessment of ticks in a hot cave in the Amazon reveals that the genus Antricola (Ixodida: Argasidae) has the highest cave tick population density on planet.Experimental & applied acarology · 2026Article
- A framework for optimising arthropod DNA quality and quantity for modern sequencing tools using hard ticks (Ixodidae).Parasitology research · 2026Article
- MitoCOMON: whole mitochondrial DNA sequencing by primer design and long overlapping amplicon assembly.BMC genomics · 2025Article
- Mitochondrial genome of Bactrocera fruit flies (Tephritidae: Dacini): features, structure, and significance for diagnosis.BMC genomics · 2025Article
- Deep sequencing of 16mSystems · 2025Article
- Genome report: whole-genome assembly of the relapsing fever tick Ornithodoros turicata Dugès (Acari: Argasidae).G3 (Bethesda, Md.) · 2025Article
- Tick genomics through a Nanopore: a low-cost approach for tick genomics.BMC genomics · 2025Article
- Phylogenetic taxonomy of the Zambian Anopheles coustani group using a mitogenomics approach.Malaria journal · 2025Article
- Mitogenomes of mosquito species of Harris County in Texas.Scientific reports · 2025Article
- Identification and characterization of a novel Pavlovskyella (Acari: Argasidae) from Chile, parasite of the culpeo fox (Lycalopex culpaeus).Journal of medical entomology · 2025Article
- The complete mitochondrial genome analysis ofOpen life sciences · 2025Article
- Description of a newParasitology · 2024Article
- Mitochondrial genome and nuclear ribosomal RNA analysis placeParasitology · 2024Article
- Novel Rickettsia and host records for argasid ticks, including Alveonasus cooleyi, on wild mammals in Baja California, Mexico.Experimental & applied acarology · 2024Article
- New insights into the molecular phylogeny, biogeographical history, and diversification of Amblyomma ticks (Acari: Ixodidae) based on mitogenomes and nuclear sequences.Parasites & vectors · 2024Article
- American mitogenome reference for the tropical brown dog tick,Current research in parasitology & vector-borne diseases · 2024Article
- Identification of non-model mammal species using the MinION DNA sequencer from Oxford Nanopore.PeerJ · 2024Article
Corrections and comments
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Authors and funding
18 authors at 14 institutions in 8 countries.
Funding
Abstract
The mitochondrial genome (mitogenome) has proven to be important for the taxonomy, systematics, and population genetics of ticks. However, current methods to generate mitogenomes can be cost-prohibitive at scale. To address this issue, we developed a cost-effective approach to amplify and sequence the whole mitogenome of individual tick specimens. Using two different primer sites, this approach generated two full-length mitogenome amplicons that were sequenced using the Oxford Nanopore Technologies' Mk1B sequencer. We used this approach to generate 85 individual tick mitogenomes from samples comprised of the three tick families, 11 genera, and 57 species. Twenty-six of these species did not have a complete mitogenome available on GenBank prior to this work. We benchmarked the accuracy of this approach using a subset of samples that had been previously sequenced by low-coverage Illumina genome skimming. We found our assemblies were comparable or exceeded the Illumina method, achieving a median sequence concordance of 99.98%. We further analyzed our mitogenome dataset in a mitophylogenomic analysis in the context of all three tick families. We were able to sequence 72 samples in one run and achieved a cost/sample of ~ $10 USD. This cost-effective strategy is applicable for sample identification, taxonomy, systematics, and population genetics for not only ticks but likely other metazoans; thus, making mitogenome sequencing equitable for the wider scientific community.
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Registered trials
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