Evidence map›Paper›PMID 36368331›Full record

ArticleStem cell reports2022

Laminin111-based defined culture promoting self-renewing human pluripotent stem cells with properties of the early post-implantation epiblast.

Michal Gropp, Ithai Waldhorn, Yaniv Gil, Debora Steiner, Tikva Tako Turetsky, Yoav Smith, Ofra Sabag, Tal Falick-Michaeli, Sharona Even Ram, Benjamin E Reubinoff

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.4field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Pluripotent cell states and fates in human embryo models.Development (Cambridge, England) · 2025
    Review
  3. Article
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Michal GroppThe Hadassah Stem Cell Research Center, Goldyne Savad Institute of Gene Therapy, Hadassah-Hebrew University Medical Center, 91120 Jerusalem, Israel.
Ithai WaldhornThe Hadassah Stem Cell Research Center, Goldyne Savad Institute of Gene Therapy, Hadassah-Hebrew University Medical Center, 91120 Jerusalem, Israel.
Yaniv GilThe Hadassah Stem Cell Research Center, Goldyne Savad Institute of Gene Therapy, Hadassah-Hebrew University Medical Center, 91120 Jerusalem, Israel.
Debora SteinerThe Hadassah Stem Cell Research Center, Goldyne Savad Institute of Gene Therapy, Hadassah-Hebrew University Medical Center, 91120 Jerusalem, Israel.
Tikva Tako TuretskyThe Hadassah Stem Cell Research Center, Goldyne Savad Institute of Gene Therapy, Hadassah-Hebrew University Medical Center, 91120 Jerusalem, Israel.
Yoav SmithThe Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem, Israel.
Ofra SabagDepartment of Developmental Biology and Cancer Research, Faculty of Medicine, Hebrew University of Jerusalem, Israel.
Tal Falick-MichaeliRubin Chair in Medical Science, Department of Developmental Biology & Cancer Research, IMRIC, Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem, Israel.
Sharona Even RamThe Hadassah Stem Cell Research Center, Goldyne Savad Institute of Gene Therapy, Hadassah-Hebrew University Medical Center, 91120 Jerusalem, Israel.
Benjamin E ReubinoffThe Hadassah Stem Cell Research Center, Goldyne Savad Institute of Gene Therapy, Hadassah-Hebrew University Medical Center, 91120 Jerusalem, Israel; Department of Obstetrics and Gynecology, Hadassah-Hebrew University Medical Center, 91120 Jerusalem, Israel. Electronic address: benr@hadassah.org.il.
Hadassah Medical Center · ILHebrew University of Jerusalem · IL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In the mammalian embryo, a formative pluripotent phase is proposed to exist at the early post-implantation period, during the transition from the pre-implantation naive-to the post-implantation primed-epiblast. By recapitulating a laminin component of the extracellular matrix niche during embryonic formative transition, and defined culture conditions, we generated cultures highly enriched for self-renewing human pluripotent stem cells (hPSCs), exhibiting properties of early post-implantation epiblast cells. These hPSCs display post-implantation-epiblast gene expression profiles. FGF and TGF-β signaling maintain their self-renewal for multiple passages. They have inactive canonical Wnt signaling, do not express primitive streak markers, and are competent to initiate differentiation toward germline and somatic fates. hPSCs exhibiting early post-implantation epiblast properties may shed light on human embryonic PSCs development and may serve for initiating somatic and germ cell specification.

Indexed as

Germ LayersPluripotent Stem CellsAnimalsCell DifferentiationEmbryo, MammalianHumansMammalsPrimitive StreakWnt Signaling Pathwaycanonical Wnt signalingearly post-implantation epiblastformative pluripotencygerm cell specificationhuman pluripotency stateshuman pluripotent stem cellslaminin-111

Identifiers

PMID36368331
PMCPMC9768580
OpenAlexW4308834894

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.