Evidence map›Paper›PMID 36366221›Full record

ArticleSensors (Basel, Switzerland)2022

Mediator Microbial Biosensor Analyzers for Rapid Determination of Surface Water Toxicity.

Anna Kharkova, Vyacheslav Arlyapov, Anastasia Medvedeva, Roman Lepikash, Pavel Melnikov, Anatoly Reshetilov

Abstract read
In one paragraph

Article in Sensors (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
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  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Anna KharkovaDepartment of Chemistry, Tula State University, 92 Lenin Avenue, Tula 300012, Russia.ORCID 0000-0002-0451-8080
Vyacheslav ArlyapovDepartment of Chemistry, Tula State University, 92 Lenin Avenue, Tula 300012, Russia.ORCID 0000-0002-5449-7353
Anastasia MedvedevaDepartment of Chemistry, Tula State University, 92 Lenin Avenue, Tula 300012, Russia.
Roman LepikashDepartment of Chemistry, Tula State University, 92 Lenin Avenue, Tula 300012, Russia.
Pavel MelnikovM.V. Lomonosov Institute of Fine Chemical Technologies, MIREA-Russian Technological University, Prospect Vernadskogo 86, Moscow 119571, Russia.ORCID 0000-0001-8438-1952
Anatoly ReshetilovG.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center "Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences" (FRC PSCBR), Russian Academy of Sciences, 5 Nauki Avenue, Moscow 142290, Russia.ORCID 0000-0002-9607-253X

Funding

President of the Russian Federation for the State Support of Young PhD Scientists grant No МК-4815.2022.1.4
6 · The paper itself

Abstract

Microbial mediator biosensors for surface water toxicity determination make it possible to carry out an early assessment of the environmental object’s quality without time-consuming standard procedures based on standard test-organisms, and provide broad opportunities for receptor element modifying depending on the required operational parameters analyzer. Four microorganisms with broad substrate specificity and nine electron acceptors were used to form a receptor system for toxicity assessment. Ferrocene was the most effective mediator according to its high rate constant of interaction with the microorganisms (0.33 ± 0.01 dm3/(g × s) for yeast Saccharomyces cerevisiae). Biosensors were tested on samples containing four heavy metal ions (Cu2+, Zn2+, Pb2+, Cd2+), two phenols (phenol and p-nitrophenol), and three natural water samples. The «ferrocene- Escherichia coli» and «ferrocene-Paracoccus yeei, E. coli association» systems showed good operational stability with a relative standard deviation of 6.9 and 7.3% (14 measurements) and a reproducibility of 7 and 5.2% using copper (II) ions as a reference toxicant. Biosensor analysis with these systems was shown to highly correlate with the results of the standard method using Chlorella algae as a test object. Developed biosensors allow for a valuation of the polluted natural water’s impact on the ecosystem via an assessment of the influence on bacteria and yeast in the receptor system. The systems could be used in toxicological monitoring of natural waters.

Indexed as

Biosensing TechniquesChlorellaMetals, HeavyWater Pollutants, ChemicalEcosystemEscherichia coliFerrous CompoundsMetallocenesReproducibility of ResultsSaccharomyces cerevisiaeWaterferroceneFerrous CompoundsMetallocenesMetals, HeavyWaterWater Pollutants, Chemicalbioelectrocatalysisearly-warning instrumentelectroactive biofilmselectron transport mediatormediator biosensorwater toxicity

Identifiers

PMID36366221
PMCPMC9655160

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.