Evidence map›Paper›PMID 36362184›Full record

ArticleInternational journal of molecular sciences2022

Generation of Functional Immortalized Human Corneal Stromal Stem Cells.

Aurelie Dos Santos, Ning Lyu, Alis Balayan, Rob Knight, Katherine Sun Zhuo, Yuzhao Sun, Jianjiang Xu, Martha L Funderburgh, James L Funderburgh, Sophie X Deng

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.3field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Aurelie Dos SantosStein Eye Institute, University of California Los Angeles, Los Angeles, CA 90095, USA.ORCID 0000-0001-7323-835X
Ning LyuStein Eye Institute, University of California Los Angeles, Los Angeles, CA 90095, USA.
Alis BalayanStein Eye Institute, University of California Los Angeles, Los Angeles, CA 90095, USA.
Rob KnightStein Eye Institute, University of California Los Angeles, Los Angeles, CA 90095, USA.
Katherine Sun ZhuoHuman Biology Society, University of California Los Angeles, Los Angeles, CA 90095, USA.
Yuzhao SunStein Eye Institute, University of California Los Angeles, Los Angeles, CA 90095, USA.
Jianjiang XuDepartment of Ophthalmology and Visual Science, Eye & ENT Hospital, Shanghai Medical College of Fudan University, Shanghai 200031, China.
Martha L FunderburghEye and Ear Institute, University of Pittsburgh, Pittsburgh, PA 15260, USA.
James L FunderburghEye and Ear Institute, University of Pittsburgh, Pittsburgh, PA 15260, USA.
Sophie X DengStein Eye Institute, University of California Los Angeles, Los Angeles, CA 90095, USA.
University of California, Los Angeles · USShanghai Medical College of Fudan University · CNUniversity of Pittsburgh · US

Funding

Pilot and Feasibility ProgramP30DK041301 · NIDDK · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ROZENGURT, JUAN ENRIQUE · 1990 to 2019
$18.0M
China Scholarship Council 201906100167NIDDK NIH HHS P30 DK041301UCLA Integrated Molecular Technologies Core CURE/P30 DK041301
6 · The paper itself

Abstract

In addition to their therapeutic potential in regenerative medicine, human corneal stromal stem cells (CSSCs) could serve as a powerful tool for drug discovery and development. Variations from different donors, their isolation method, and their limited life span in culture hinder the utility of primary human CSSCs. To address these limitations, this study aims to establish and characterize immortalized CSSC lines (imCSSC) generated from primary human CSSCs. Primary CSSCs (pCSSC), isolated from human adult corneoscleral tissue, were transduced with ectopic expression of hTERT, c-MYC, or the large T antigen of the Simian virus 40 (SV40T) to generate imCSSC. Cellular morphology, proliferation capacity, and expression of CSSCs specific surface markers were investigated in all cell lines, including

Indexed as

Corneal StromaStromal CellsAdultCell DifferentiationCell LineHumansStem Cellsbioengineered cell linecorneal stromal stem cellsimmortalization

Identifiers

PMID36362184
PMCPMC9657819
OpenAlexW4309091801

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.