Evidence map›Paper›PMID 36362119›Full record

ArticleInternational journal of molecular sciences2022

Design and Characterization of In-One Protease-Esterase PluriZyme.

Laura Fernandez-Lopez, Sergi Roda, Jose L Gonzalez-Alfonso, Francisco J Plou, Víctor Guallar, Manuel Ferrer

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.5field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Cold-Adapted Proteases: An Efficient and Energy-Saving Biocatalyst.International journal of molecular sciences · 2023
    Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Laura Fernandez-LopezDepartment of Applied Biocatalysis, ICP, CSIC, 28049 Madrid, Spain.ORCID 0000-0001-8861-3191
Sergi RodaDepartment of Life Sciences, Barcelona Supercomputing Center (BSC), 08034 Barcelona, Spain.ORCID 0000-0002-0174-7435
Jose L Gonzalez-AlfonsoDepartment of Applied Biocatalysis, ICP, CSIC, 28049 Madrid, Spain.ORCID 0000-0002-3396-7985
Francisco J PlouDepartment of Applied Biocatalysis, ICP, CSIC, 28049 Madrid, Spain.ORCID 0000-0003-0831-893X
Víctor GuallarDepartment of Life Sciences, Barcelona Supercomputing Center (BSC), 08034 Barcelona, Spain.ORCID 0000-0002-4580-1114
Manuel FerrerDepartment of Applied Biocatalysis, ICP, CSIC, 28049 Madrid, Spain.ORCID 0000-0003-4962-4714
Barcelona Supercomputing Center · ESInstitució Catalana de Recerca i Estudis Avançats · ES

Funding

Agencia Estatal CSIC 2020AEP061European Union 101000327European Union 101000607European Union ("NextGenerationEU/PRTR") PDC2021-121534-I00Ministerio de Ciencia e Innovación, Agencia Estatal de Investigación (AEI) PID2020-112758RB-I00 (M.F.), PID2019-106370RB-I00 (V.G.), PDC2021-121534-I00 (M.F.), and PID2019-105838RB-C31 (F.J.P.)
6 · The paper itself

Abstract

Proteases are abundant in prokaryotic genomes (~10 per genome), but their recovery encounters expression problems, as only 1% can be produced at high levels; this value differs from that of similarly abundant esterases (1-15 per genome), 50% of which can be expressed at good levels. Here, we design a catalytically efficient artificial protease that can be easily produced. The

Indexed as

EsterasesPeptide HydrolasesCatalytic DomainEndopeptidasesEstersHydrogen-Ion ConcentrationEndopeptidasesEsterasesEstersPeptide Hydrolasescascade reactioncomputational chemistryesterasePluriZymeproteaseprotein engineering

Identifiers

PMID36362119
PMCPMC9655419
OpenAlexW4307964116

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.