ReviewInternational journal of molecular sciences2022
Pros and Cons of In Vitro Methods for Circular RNA Preparation.
Review in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed.
- Target site selection and P1 engineering enable highly efficient circular RNA production via end-to-end self-targeting and splicing.Nucleic acids research · 2026Article
- Review
- Circular RNA as a New Vaccine Platform: Considerations, Challenges, and Perspectives.Vaccines · 2026Review
- RNA chemistry and therapeutics.Nature reviews. Drug discovery · 2025Review
- Circular RNAs in hepatitis B virus-induced hepatocellular carcinoma: A comprehensive review and recent advances.Genes & diseases · 2025Review
- Circular RNA vaccines: Pioneering the next-gen cancer immunotherapy.Cancer pathogenesis and therapy · 2025Review
- Potential of Circular RNAs (circRNAs) Neoantigen Vaccines in Tumor Immunotherapy.Current protein & peptide science · 2025Review
- Analytical Methods to Evaluate RNA Circularization Efficiency.Electrophoresis · 2024Article
- Review
- Circular RNAs in Cardiovascular Diseases: Molecular Mechanisms, Therapeutic Advances, and Innovations.Genes · 2024Review
- Advances and applications of RNA vaccines in tumor treatment.Molecular cancer · 2024Review
- In Vitro Self-Circularization Methods Based on Self-Splicing Ribozyme.International journal of molecular sciences · 2024Review
- Advances in Engineering Circular RNA Vaccines.Pathogens (Basel, Switzerland) · 2024Review
- CircRNA as an Achilles heel of cancer: characterization, biomarker and therapeutic modalities.Journal of translational medicine · 2024Review
- A cis-acting ligase ribozyme generates circular RNA in vitro for ectopic protein functioning.Nature communications · 2024Article
- Effective synthesis of circRNA via a thermostable T7 RNA polymerase variant as the catalyst.Frontiers in bioengineering and biotechnology · 2024Article
- Efficient circular RNA engineering by end-to-end self-targeting and splicing reaction usingMolecular therapy. Nucleic acids · 2023Article
- Circular RNA vaccine in disease prevention and treatment.Signal transduction and targeted therapy · 2023Review
- Editorial to the IJMS Special Issue on "ncRNAS in Therapeutics".International journal of molecular sciences · 2023Article
- Cap-Independent Circular mRNA Translation Efficiency.Vaccines · 2023Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
mRNA is gaining success as a new therapeutic agent and vaccine. However, mRNA has limitations in stability. To overcome the shortcomings of mRNA, circular RNA is emerging as a new modality. In this review, several current methods of manufacturing circular RNA in vitro are introduced and their advantages and disadvantages are reviewed. Furthermore, this study discusses which fields and directions of research and development are needed for the increase in the efficacy and productivity of circular RNA as a therapeutic agent and vaccine formulation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.