Evidence map›Paper›PMID 36361666›Full record

ArticleInternational journal of molecular sciences2022

Prenatal Maternal Antibiotics Treatment Alters the Gut Microbiota and Immune Function of Post-Weaned Prepubescent Offspring.

Abdullah M Madany, Heather K Hughes, Paul Ashwood

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed, 1 pooled it
3.2field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 1 synthesis or guideline pooled it, 16 citations in OpenAlex.

  1. Pooled it
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  9. The importance of gut microbiome in the perinatal period.European journal of pediatrics · 2024
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Abdullah M MadanyDepartment of Psychiatry and Behavioral Sciences, University of California at Davis, 2230 Stockton Blvd., Sacramento, CA 95817, USA.
Heather K HughesThe M.I.N.D. Institute, University of California at Davis, 2825 50th Street, Sacramento, CA 95817, USA.
Paul AshwoodThe M.I.N.D. Institute, University of California at Davis, 2825 50th Street, Sacramento, CA 95817, USA.ORCID 0000-0002-4255-1893
University of California, Davis · US

Funding

Interdisciplinary Training for Autism Researchers(RMI)T32MH073124 · NIMH · UNIVERSITY OF CALIFORNIA DAVIS · PI Stephen Charles Noctor, Christine Wu Nordahl · 2004 to 2026
$6.8M
Immune regulation and gastrointestinal co-morbidity in autism spectrum disordersR01HD090214 · NICHD · UNIVERSITY OF CALIFORNIA AT DAVIS · PI ASHWOOD, PAUL · 2018 to 2022
$1.9M
Autism Research Institute 123456NICHD NIH HHS R01 HD090214NIH HHS 123456NIH HHS 90120NIMH NIH HHS T32 MH073124
6 · The paper itself

Abstract

This study aimed to investigate the immediate and continual perturbation to the gut microbiota of offspring in the weeks post-weaning and how these may be modulated by treating pregnant C57BL/6J dams with antibiotics (ABX). We used a broad-spectrum antibiotic cocktail consisting of ampicillin 1 mg/mL, neomycin 1 mg/mL, and vancomycin 0.5 mg/mL, or vancomycin 0.5 mg/mL alone, administered ad-lib orally to dams via drinking water during gestation and stopped after delivery. We analyzed the gut microbiota of offspring, cytokine profiles in circulation, and the brain to determine if there was evidence of a gut-immune-brain connection. Computationally predicted metabolic pathways were calculated from 16s rRNA sequencing data. ABX treatment can negatively affect the gut microbiota, including reduced diversity, altered metabolic activity, and immune function. We show that the maternal ABX-treatment continues to alter the offspring's gut microbiota diversity, composition, and metabolic pathways after weaning, with the most significant differences evident in 5-week-olds as opposed to 4-week-olds. Lower levels of chemokines and inflammatory cytokines, such as interleukin (IL)-1α and IL-2, are also seen in the periphery and brains of offspring, respectively. In conclusion, this study shows maternal antibiotic administration alters gut microbiome profiles in offspring, which undergoes a continuous transformation, from week to week, at an early age after weaning.

Indexed as

Gastrointestinal MicrobiomeAnimalsAnti-Bacterial AgentsFemaleImmunityMiceMice, Inbred C57BLPregnancyRNA, Ribosomal, 16SVancomycinWeaningAnti-Bacterial AgentsRNA, Ribosomal, 16SVancomycinantibiotics (ABX)autism spectrum disorder (ASD)BacteroidetescytokinesdysbiosisFirmicutesgut microbiotainterleukinLactobacilluslipopolysaccharide (LPS)metabolic pathwaysMuribaculaceaeneurodevelopmental disorders (NDD)prepubescentschizophreniaweaning

Identifiers

PMID36361666
PMCPMC9655507
OpenAlexW4307552374

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.