Evidence map›Paper›PMID 36359902›Full record

ArticleCells2022

LncKCNQ1OT1 Promotes the Odontoblastic Differentiation of Dental Pulp Stem Cells via Regulating hsa-miR-153-3p/RUNX2 Axis.

Xiaohui Lu, Jiawen Zhang, Yuanzhou Lu, Jing Xing, Min Lian, Guijuan Feng, Dan Huang, Chenfei Wang, Nimei Shen, Xingmei Feng

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 49% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Xiaohui LuDepartment of Stomatology, Affiliated Hospital of Nantong University, Nantong 226001, China.
Jiawen ZhangDepartment of Stomatology, Affiliated Hospital of Nantong University, Nantong 226001, China.
Yuanzhou LuDepartment of Cardiology, Tongzhou People's Hospital, Nantong 226399, China.
Jing XingDepartment of Stomatology, Affiliated Hospital of Nantong University, Nantong 226001, China.
Min LianDepartment of Stomatology, Affiliated Hospital of Nantong University, Nantong 226001, China.
Guijuan FengDepartment of Stomatology, Affiliated Hospital of Nantong University, Nantong 226001, China.
Dan HuangDepartment of Stomatology, Affiliated Hospital of Nantong University, Nantong 226001, China.
Chenfei WangDepartment of Stomatology, Affiliated Hospital of Nantong University, Nantong 226001, China.
Nimei ShenDepartment of Ophthalmology and Otorhinolaryngology, The Second Affiliated Hospital of Nantong University, Nantong 226001, China.
Xingmei FengDepartment of Stomatology, Affiliated Hospital of Nantong University, Nantong 226001, China.
Nantong University · CNAffiliated Hospital of Nantong University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study aimed to explore the role of LncKCNQ1OT1/hsa-miR-153-3p/RUNX2 in the odontoblastic differentiation of human dental pulp stem cells (DPSCs) and its possible mechanism. The expression of LncKCNQ1OT1, hsa-miR-153-3p, and RUNX2 in the odontoblastic differentiation was detected by qRT-PCR. Interaction between LncKCNQ1OT1 and hsa-miR-153-3p and interaction between hsa-miR-153-3p and RUNX2 were detected by dual-luciferase assay. The cell viability of DPSCs was detected by CCK-8, and the effect of LncKCNQ1OT1 and hsa-miR-153-3p on the odontoblastic differentiation of DPSCs was observed by alizarin red staining, alkaline phosphatase (ALP) activity assay, and Western blot for RUNX2, DSPP, and DMP-1. The results showed, during odontoblastic differentiation of DPSCs, the expression of LncKCNQ1OT1 increased, hsa-miR-153-3p expression decreased, and RUNX2 expression increased. Dual-luciferase assay showed that LncKCNQ1OT1 sponges hsa-miR-153-3p and hsa-miR-153-3p targets on RUNX2. After LncKCNQ1OT1 and hsa-miR-153-3p expressions of DPSCs were changed, the cell viability was not notably changed, but the odontoblastic differentiation was notably changed, which was confirmed with Alizarin Red staining, ALP activity, and Western blot for RUNX2, DSPP, and DMP-1. The results indicate that LncKCNQ1OT1 promotes the odontoblastic differentiation of DPSCs via regulating hsa-miR-153-3p/RUNX2 axis, which may provide a therapeutic clue for odontogenesis.

Indexed as

Core Binding Factor Alpha 1 SubunitDental PulpAnthraquinonesCell DifferentiationHumansMicroRNAsPotassium Channels, Voltage-GatedStem CellsalizarinAnthraquinonesCore Binding Factor Alpha 1 SubunitKCNQ1OT1 long non-coding RNA, humanMicroRNAsMIRN153 microRNA, humanPotassium Channels, Voltage-GatedRUNX2 protein, humandental pulp stem cellshsa-miR-153-3pLncKCNQ1OT1odontoblastic differentiationRUNX2

Identifiers

PMID36359902
PMCPMC9654545
OpenAlexW4308456496

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.