Evidence map›Paper›PMID 36359412›Full record

ArticleDiagnostics (Basel, Switzerland)2022

Improving the Detection Sensitivity of a New Rapid Diagnostic Technology for Severe Acute Respiratory Syndrome Coronavirus 2 Using a Trace Amount of Saliva.

Reiko Tokuyama-Toda, Chika Terada-Ito, Masaaki Muraoka, Toshikatsu Horiuchi, Tsuyoshi Amemiya, Airi Fukuoka, Yoshiki Hamada, Yusuke Takebe, Takashi Ogawa, Seiko Fujii and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Diagnostics (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Reiko Tokuyama-TodaDepartment of Oral Medicine and Stomatology, School of Dental Medicine, Tsurumi University, 2-1-3, Tsurumi, Tsurumi-ku, Yokohama City 230-8501, Kanagawa, Japan.ORCID 0000-0003-3932-5913
Chika Terada-ItoDepartment of Oral Medicine and Stomatology, School of Dental Medicine, Tsurumi University, 2-1-3, Tsurumi, Tsurumi-ku, Yokohama City 230-8501, Kanagawa, Japan.ORCID 0000-0001-5380-5599
Masaaki MuraokaCertified Non-Profit Organization Biomedical Science Association, 2-20-8, Kamiosaki, Shinagawa-ku 141-0021, Tokyo, Japan.ORCID 0000-0002-2926-5374
Toshikatsu HoriuchiDepartment of Oral and Maxillofacial Surgery, Saiseikai Yokohamashi Tobu Hospital, 3-6-1, Shimosueyoshi, Tsurumi-ku, Yokohama City 230-8765, Kanagawa, Japan.
Tsuyoshi AmemiyaDepartment of Oral and Maxillofacial Surgery, Saiseikai Yokohamashi Tobu Hospital, 3-6-1, Shimosueyoshi, Tsurumi-ku, Yokohama City 230-8765, Kanagawa, Japan.
Airi FukuokaDepartment of Oral and Maxillofacial Surgery, Saiseikai Yokohamashi Tobu Hospital, 3-6-1, Shimosueyoshi, Tsurumi-ku, Yokohama City 230-8765, Kanagawa, Japan.
Yoshiki HamadaDepartment of Oral and Maxillofacial Surgery, School of Dental Medicine, Tsurumi University, 2-1-3, Tsu-rumi, Tsurumi-ku, Yokohama City 230-8501, Kanagawa, Japan.
Yusuke TakebeDepartment of Oral Medicine and Stomatology, School of Dental Medicine, Tsurumi University, 2-1-3, Tsurumi, Tsurumi-ku, Yokohama City 230-8501, Kanagawa, Japan.
Takashi OgawaDepartment of Oral and Maxillofacial Surgery, Tokyo Medical University Hachioji Medical Center, 1163, Tatemachi, Hachioji City 193-0998, Tokyo, Japan.
Seiko FujiiDepartment of Oral and Maxillofacial Surgery, Shin-Yurigaoka General Hospital, 255, Furusawatsuko, Asao-ku, Kawasaki City 215-0026, Kanagawa, Japan.
Toshihiro KikutaDepartment of Oral and Maxillofacial Surgery, Shin-Yurigaoka General Hospital, 255, Furusawatsuko, Asao-ku, Kawasaki City 215-0026, Kanagawa, Japan.
Shunsuke SejimaCertified Non-Profit Organization Biomedical Science Association, 2-20-8, Kamiosaki, Shinagawa-ku 141-0021, Tokyo, Japan.
Kazuhito SatomuraDepartment of Oral Medicine and Stomatology, School of Dental Medicine, Tsurumi University, 2-1-3, Tsurumi, Tsurumi-ku, Yokohama City 230-8501, Kanagawa, Japan.ORCID 0000-0003-2159-1279

Funding

Japanese Association for Dental Science, Sponsored Research 2021-A-5JSPS KAKENHI 20K10150 and 20K10104
6 · The paper itself

Abstract

The early diagnosis and isolation of infected individuals with coronavirus disease 2019 (COVID-19) remain important. Although quantitative polymerase chain reaction (qPCR) testing is considered the most accurate test available for COVID-19 diagnosis, it has some limitations, such as the need for specialized laboratory technicians and a long turnaround time. Therefore, we have established and reported a rapid diagnostic method using a small amount of saliva as a sample using a lightweight mobile qPCR device. This study aimed to improve the existing method and increase the detection sensitivity and specificity. The detection specificity of CDC N1 and N2 was examined by improving qPCR reagents and polymerase chain reaction conditions for the previously reported method. Furthermore, the feasibility of detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral RNA was examined using both the previous method and the improved method in patients with COVID-19. The results showed that the improved method increased the specificity and sensitivity. This improved method is useful for the rapid diagnosis of SARS-CoV-2.

Indexed as

COVID-19diagnostic technologymobile qPCR devicemouthwashsalivaSARS-CoV-2

Identifiers

PMID36359412
PMCPMC9689209

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.