ArticleAnimals : an open access journal from MDPI2022
Integrative Analysis of miRNA-mRNA in Ovarian Granulosa Cells Treated with Kisspeptin in Tan Sheep.
Article in Animals : an open access journal from MDPI, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 10 citations in OpenAlex.
- Longitudinal Genome-Wide Association Study for Female Fertility Traits in German Holstein Cattle.Animal genetics · 2026Article
- Manipulating seasonality by using PMSG and Kisspeptin hormones and the impact of the MTNR1A gene on reproduction efficiency in ewes.Open veterinary journal · 2025Article
- Seasonal influence on miRNA expression dynamics of extracellular vesicles in equine follicular fluid.Journal of animal science and biotechnology · 2024Article
- Does the miR-105-1-Kisspeptin Axis Promote Ovarian Cell Functions?Reproductive sciences (Thousand Oaks, Calif.) · 2024Article
- Dynamic Expression of Follicle-Stimulating Hormone and Estrogen mRNA Receptors Associated with microRNAsAnimals : an open access journal from MDPI · 2024Article
- Expression profiles of circulating miRNAs in an endangered Piedmontese sheep breed during the estrus cycle.Frontiers in veterinary science · 2024Article
- Extracellular vesicle-microRNAs mediated response of bovine ovaries to seasonal environmental changes.Journal of ovarian research · 2023Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
Kisspeptin is a peptide hormone encoded by the kiss-1 gene that regulates animal reproduction. Our studies revealed that kisspeptin can regulate steroid hormone production and promote cell proliferation in ovarian granulosa cells of Tan sheep, but the mechanism has not yet been fully understood. We speculated that kisspeptin might promote steroid hormone production and cell proliferation by mediating the expression of specific miRNA and mRNA in granulosa cells. Accordingly, after granulosa cells were treated with kisspeptin, the RNA of cells was extracted to construct a cDNA library, and miRNA-mRNA sequencing was performed. Results showed that 1303 expressed genes and 605 expressed miRNAs were identified. Furthermore, eight differentially expressed miRNAs were found, and their target genes were significantly enriched in progesterone synthesis/metabolism, hormone biosynthesis, ovulation cycle, and steroid metabolism regulation. Meanwhile, mRNA was significantly enriched in steroid biosynthesis, IL-17 signaling pathway, and GnRH signaling pathway. Integrative analysis of miRNA-mRNA revealed that the significantly different oar-let-7b targets eight genes, of which EGR1 (early growth response-1) might play a significant role in regulating the function of granulosa cells, and miR-10a regulates lipid metabolism and steroid hormone synthesis by targeting HNRNPD. Additionally, PPI analysis revealed genes that are not miRNA targets but crucial to other biological processes in granulosa cells, implying that kisspeptin may also indirectly regulate granulosa cell function by these pathways. The findings of this work may help understand the molecular mechanism of kisspeptin regulating steroid hormone secretion, cell proliferation, and other physiological functions in ovarian granulosa cells of Tan sheep.
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