Evidence map›Paper›PMID 36356379›Full record

ArticleNutrition (Burbank, Los Angeles County, Calif.)2023

Polyunsaturated fatty acids ameliorate renal stone-induced renal tubular damage via miR-93-5p/Pknox1 axis.

Qin Liu, Jun Tang, Zhong Chen, Lanji Wei, Jianying Chen, Zhijuan Xie

Abstract read
PubMed Publisher
In one paragraph

Article in Nutrition (Burbank, Los Angeles County, Calif.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.2field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 8 citations in OpenAlex.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Qin LiuDepartment of Nephrology, The First Affiliated Hospital of University of South China, Hengyang Medical School, University of South China, Hengyang, Hunan, P. R. China.
Jun TangDepartment of Emergency, The First Affiliated Hospital of University of South China, Hengyang Medical School, University of South China, Hengyang, Hunan, P. R. China.
Zhong ChenDepartment of Nuclear Medicine, The First Affiliated Hospital of University of South China, Hengyang Medical School, University of South China, Hengyang, Hunan, P. R. China.
Lanji WeiHealth Management Center, The Affiliated Nanhua Hospital of University of South China, Hengyang Medical School, University of South China, Hengyang, Hunan, P. R. China.
Jianying ChenDepartment of Rheumatology and Immunology, Hunan Provincial People's Hospital (Mawangdui Hospital), Changsha, Hunan, China.
Zhijuan XieDepartment of Nephrology, The First Affiliated Hospital of University of South China, Hengyang Medical School, University of South China, Hengyang, Hunan, P. R. China. Electronic address: xzj7585@163.com.
University of South China · CNFirst Affiliated Hospital of University of South China · CNHunan Provincial People's Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesPolyunsaturated fatty acids (PUFAs) can decrease the risk of calcium oxalate stone formation, which accounts for 80% of all renal stones. This study aimed to investigate the protective mechanisms of PUFAs against renal stones.

methodsUrine samples of patients with renal stones and biopsy tissue samples from patients with nephrocalcinosis were tested for miR-93-5p expression. A renal stone mouse model was established with intraperitoneal injection of glyoxylic acid, during which mice were treated with PUFAs and/or an miR-93-5p inhibitor adenovirus. Periodic acid-Schiff staining, terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick end labeling staining, oil red O staining, triacylglycerol assay, and colorimetry testing were performed to assess glycogen deposition, apoptosis, lipid accumulation, blood urea nitrogen, and serum creatinine levels, respectively. Renal proximal tubular epithelial cells (human kidney 2 [HK-2]) were subjected to gain- and loss-of-function assays before calcium-oxalate monohydrate (COM) induction and PUFA treatment. Cell counting kit 8, flow cytometry, and lactate dehydrogenase activity assays were used to examine cell viability, apoptosis, and damage. A luciferase reporter gene assay verified the interaction between miR-93-5p and Pknox1, and miR-93-5p and Pknox1 levels were assessed using a reverse transcription-quantitative polymerase chain reaction and Western blot analysis.

resultsmiR-93-5p was downregulated in clinical samples with renal stones and negatively targeted Pknox1. PUFAs increased miR-93-5p expression and reduced apoptosis, glycogen deposition, and lipid accumulation in mice with renal stones, which were annulled by miR-93-5p downregulation. PUFAs increased proliferation and diminished apoptosis, lipid accumulation, and lactate dehydrogenase activity in COM-induced HK-2 cells, which were negated by miR-93-5p inhibition. Pknox1 overexpression reversed the effect of miR-93-5p upregulation on COM-induced HK-2 cells.

conclusionsPUFAs repressed renal stone-induced renal tubular damage via the miR-93-5p/Pknox1 axis.

Indexed as

Fatty Acids, UnsaturatedHomeodomain ProteinsKidney CalculiMicroRNAsAnimalsApoptosisGlycogenHumansLactate DehydrogenasesLipidsMiceFatty Acids, UnsaturatedGlycogenHomeodomain ProteinsLactate DehydrogenasesLipidsMicroRNAsMIRN93 microRNA, humanMirn93 microRNA, mousePKNOX1 protein, humanPknox1 protein, mousemicroRNA-93-5pPknox1Polyunsaturated fatty acidsRenal stonesRenal tubular damage

Identifiers

PMID36356379
OpenAlexW4304690795

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.