Evidence map›Paper›PMID 36355292›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Co-culture of Human Articular Chondrocytes Seeded in Polyurethane Scaffolds and Human Mesenchymal Stromal Cells Encapsulated in Alginate Beads.

Teresa Z Brose, Yann D Ladner, E Johanna Kubosch, Martin J Stoddart, Angela R Armiento

Abstract read
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Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Teresa Z BroseMedical Center-Albert-Ludwigs-University, Department of Orthopedics and Trauma Surgery, Faculty of Medicine, Albert-Ludwigs-University of Freiburg, Freiburg, Germany.
Yann D LadnerRegenerative Orthopaedics Program, AO Research Institute Davos, Davos Platz, Switzerland.
E Johanna KuboschMedical Center-Albert-Ludwigs-University, Department of Orthopedics and Trauma Surgery, Faculty of Medicine, Albert-Ludwigs-University of Freiburg, Freiburg, Germany.
Martin J StoddartRegenerative Orthopaedics Program, AO Research Institute Davos, Davos Platz, Switzerland.
Angela R ArmientoRegenerative Orthopaedics Program, AO Research Institute Davos, Davos Platz, Switzerland. angela.armiento@ucb.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Co-culturing is an essential method for unravelling the importance of cross talk and cellular interaction. This chapter describes the preparation of an indirect co-culture technique based on encapsulation of chondrocytes and mesenchymal stromal cells in polyurethane scaffolds and alginate beads, respectively. This way, both cell populations can communicate through paracrine effects in the absence of cell-cell contact. Due to the mechanical properties of polyurethane, this model can be employed in mechanobiology studies. The resulting engineered cultures can provide a more realistic environment, recreating the complex joints' microenvironment and physiology.

Indexed as

Cartilage, ArticularMesenchymal Stem CellsAlginatesCells, CulturedChondrocytesCoculture TechniquesHumansPolyurethanesTissue EngineeringAlginatesPolyurethanesAlginate beadsChondrocytesCross talkIndirect co-cultureMesenchymal stromal cellsTissue engineering

Identifiers

PMID36355292

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.