ArticleMicrobiology spectrum2022
Metabolic Engineering and Adaptive Evolution for Efficient Production of l-Lactic Acid in Saccharomyces cerevisiae.
Article in Microbiology spectrum, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 31 citations in OpenAlex.
- Adaptive laboratory evolution of Lactiplantibacillus plantarum enhances furan tolerance and lactic acid production in lignocellulosic hydrolysate.Bioresources and bioprocessing · 2026Article
- Long-term adaptive laboratory evolution of Saccharomyces cerevisiae for high-titer lactic acid production at low pH.Microbial cell factories · 2025Article
- Metabolic engineering and adaptive laboratory evolution of Kluyveromyces Marxianus for lactic acid production.Microbial cell factories · 2025Article
- Sucrose-driven carbon redox rebalancing eliminates the Crabtree effect and boosts energy metabolism in yeast.Nature communications · 2025Article
- Factors Affecting D-Lactic Acid Production by FlocculantMicroorganisms · 2025Article
- Engineering Useful Microbial Species for Pharmaceutical Applications.Microorganisms · 2025Review
- Synthetic biology strategies for sustainable bioplastic production by yeasts.Journal of microbiology (Seoul, Korea) · 2025Review
- Native and Recombinant Yeast Producers of Lactic Acid: Characteristics and Perspectives.International journal of molecular sciences · 2025Review
- Metabolic engineering of Komagataella phaffii for enhanced 3-hydroxypropionic acid (3-HP) production from methanol.Journal of biological engineering · 2025Article
- Synthetic evolution ofmLife · 2025Review
- The advances in creating Crabtree-negative Saccharomyces cerevisiae and the application for chemicals biosynthesis.FEMS yeast research · 2025Review
- Recent advances in genetic engineering and chemical production in yeast species.FEMS yeast research · 2025Review
- Boosting succinic acid production of Yarrowia lipolytica at low pH through enhancing product tolerance and glucose metabolism.Microbial cell factories · 2024Article
- Just around the Corner: Advances in the Optimization of Yeasts and Filamentous Fungi for Lactic Acid Production.Journal of fungi (Basel, Switzerland) · 2024Review
- Fungal carboxylate transporters: recent manipulations and applications.Applied microbiology and biotechnology · 2023Review
- High quality de novo genome assembly of the non-conventional yeast Kazachstania bulderi describes a potential low pH production host for biorefineries.Communications biology · 2023Article
- Development of an itaconic acid production process with Ustilaginaceae on alternative feedstocks.BMC biotechnology · 2023Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
l-Lactic acid (LA) is a three-carbon hydroxycarboxylic acid with extensive applications in food, cosmetic, agricultural, pharmaceutical, and bioplastic industries. However, microbial LA production is limited by its intrinsic inefficiency of cellular metabolism. Here, pathway engineering was used to rewire the biosynthetic pathway for LA production in Saccharomyces cerevisiae by screening heterologous l-lactate dehydrogenase, reducing ethanol accumulation, and introducing a bacterial acetyl coenzyme A (acetyl-CoA) synthesis pathway. To improve its intrinsic efficiency of LA export, transporter engineering was conducted by screening the monocarboxylate transporters and then strengthening the capacity of LA export, leading to LA production up to 51.4 g/L. To further enhance its intrinsic efficiency of acid tolerance, adaptive evolution was adopted by cultivating yeast cells with a gradual increase in LA levels during 12 serial subcultures, resulting in a 17.5% increase in LA production to 60.4 g/L. Finally, the engineered strain S.c-NO.2-100 was able to produce 121.5 g/L LA, with a yield of up to 0.81 g/g in a 5-L batch bioreactor. The strategy described here provides a guide for developing efficient cell factories for the production of the other industrially useful organic acids.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.