Evidence map›Paper›PMID 36353718›Full record

ArticleBio-protocol2022

Single Protein Detection and Imaging with Evanescent Scattering Microscopy.

Pengfei Zhang, Lei Zhou, Rui Wang, Xinyu Zhou, Jiapei Jiang, Zijian Wan, And Shaopeng Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Pengfei ZhangBiodesign Center for Bioelectronics and Biosensors, Arizona State University, Tempe, USA.
Lei ZhouBiodesign Center for Bioelectronics and Biosensors, Arizona State University, Tempe, USA.
Rui WangBiodesign Center for Bioelectronics and Biosensors, Arizona State University, Tempe, USA.
Xinyu ZhouBiodesign Center for Bioelectronics and Biosensors, Arizona State University, Tempe, USA.
Jiapei JiangBiodesign Center for Bioelectronics and Biosensors, Arizona State University, Tempe, USA.
Zijian WanBiodesign Center for Bioelectronics and Biosensors, Arizona State University, Tempe, USA.
And Shaopeng WangBiodesign Center for Bioelectronics and Biosensors, Arizona State University, Tempe, USA.

Funding

Quantitative label-free imaging of membrane protein interaction kinetics on cellsR01GM107165 · NIGMS · ARIZONA STATE UNIVERSITY-TEMPE CAMPUS · PI WANG, SHAOPENG · 2014 to 2021
$2.2M
NIGMS NIH HHS R01 GM107165
6 · The paper itself

Abstract

Single-molecule measurements provide statistical distributions of molecular properties, in addition to the ensemble averages. Evanescent detection approaches have been widely used for single-molecule detection because the evanescent field can significantly enhance the light-analyte interaction and reduce the background noise. However, current evanescent single-molecule detection systems mostly require specially designed sensing components. Here, we show that single proteins can be imaged on a plain cover glass surface by detecting the evanescent waves scattered by the target molecules. This allows us to quantify the protein-antibody interactions at the single-molecule level. This protocol describes a label-free single-molecule imaging approach with conventional consumables and may pave the road for detecting single molecules with commercial optical microscopy.

Indexed as

Cover glassEvanescent fieldScattering microscopySingle-molecule imagingSingle proteinTotal internal reflection

Identifiers

PMID36353718
PMCPMC9606452

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.