ArticlePlant communications2023
A Nimble Cloning-compatible vector system for high-throughput gene functional analysis in plants.
Article in Plant communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed.
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- Molecular cloning and defence functional analysis ofFrontiers in plant science · 2026Article
- Integrated BSA-Seq and WGCNA Analyses Reveal Candidate Genes Associated with Winter Bud Dormancy Maintenance in Fruit Mulberry (Current issues in molecular biology · 2025Article
- miR398-Dirigent Module Negatively Regulates Cassava Resistance to Cassava Common Mosaic Virus by Affecting the Cell Wall Lignification and Thickness.Molecular plant pathology · 2025Article
- An all-in-one plant virus-based vector toolkit for streamlined gene silencing, overexpression, and genome editing.Plant communications · 2025Article
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- Functional Characterization of Anthocyanin Biosynthesis-RelatedPlants (Basel, Switzerland) · 2025Article
- Genome-wide identification of Morus notabilis Aquaporin gene family and differential expression of plasma membrane intrinsic proteins in response to Ralstonia pseudosolanacearum infection.BMC plant biology · 2025Article
- Identification of thePlants (Basel, Switzerland) · 2025Article
- Two Terpene Synthases Are Involved in Multiple Sesquiterpene Biosynthesis in the Woody Vegetable,International journal of molecular sciences · 2025Article
- Efficient gene editing of a model fern species through gametophyte-based transformation.Plant physiology · 2024Article
- Functional analysis ofMolecular breeding : new strategies in plant improvement · 2024Article
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- Comprehensive Analysis ofInternational journal of molecular sciences · 2023Article
- Molecular characterization of oleosin genes in Cyperus esculentus, a Cyperaceae plant producing oil in underground tubers.Plant cell reports · 2023Article
- Time-Series Transcriptome Analysis Reveals the Molecular Mechanism of Ethylene Reducing Cold Sensitivity of Postharvest 'Huangguan' Pear.International journal of molecular sciences · 2023Article
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Authors and funding
6 authors.
Funding
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Abstract
Plant expression vectors are essential tools for gene functional analysis and molecular plant breeding. The gene of interest is transferred to the vector by molecular cloning technology. Nimble Cloning is a newly developed molecular cloning method with the advantages of simplicity, efficiency, and standardization. In this study, we developed a "pNC" vector system that contains 55 Nimble Cloning-compatible vectors for functional analysis of genes in plants. These vectors contain the NC frame flanked by unique adapters for one-step and standardized Nimble Cloning. We demonstrate that the pNC vectors are convenient and effective for the functional analysis of plant genes, including the study of gene ectopic expression, protein subcellular localization, protein-protein interaction, gene silencing (RNAi), virus-induced gene silencing, promoter activity, and CRISPR-Cas9-mediated genome editing. The "pNC" vector system represents a high-throughput toolkit that can facilitate the large-scale analysis of plant functional genomics.
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