Evidence map›Paper›PMID 36350697›Full record

ArticleMolecular biology of the cell2023

Aurora A phosphorylates Ndel1 to reduce the levels of Mad1 and NuMA at spindle poles.

Paweł Ł Janczyk, Eliza Żyłkiewicz, Henry De Hoyos, Thomas West, Daniel R Matson, Won-Chan Choi, Heather M Raimer Young, Zygmunt S Derewenda, P Todd Stukenberg

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular biology of the cell, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Aurora kinases: Generators of spatial control during mitosis.Frontiers in cell and developmental biology · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Paweł Ł JanczykDepartment of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA 22903.
Eliza ŻyłkiewiczDepartment of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA 22903.
Henry De HoyosDepartment of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA 22903.
Thomas WestDepartment of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA 22903.
Daniel R MatsonDepartment of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA 22903.
Won-Chan ChoiDepartment of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville, VA 22903.
Heather M Raimer YoungDepartment of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA 22903.
Zygmunt S DerewendaDepartment of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville, VA 22903.
P Todd StukenbergDepartment of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA 22903.
University of Virginia · US

Funding

Women's Oncology Program - WONP30CA044579 · NCI · UNIVERSITY OF VIRGINIA CHARLOTTESVILLE · PI Jay William FOX · 1987 to 2026
$72.1M
TRAINING IN CELL AND MOLECULAR BIOLOGYT32GM008136 · NIGMS · UNIVERSITY OF VIRGINIA CHARLOTTESVILLE · PI STUKENBERG, P. TODD · 1985 to 2020
$8.5M
Molecular Mechanisms of Neuronal MigrationsR01NS036267 · NINDS · UNIVERSITY OF VIRGINIA CHARLOTTESVILLE · PI DEREWENDA, ZYGMUNT S · 1998 to 2011
$3.4M
Mechanisms of mitotic regulationR35GM148181 · NIGMS · UNIVERSITY OF VIRGINIA · PI P. TODD STUKENBERG · 2023 to 2026
$2.9M
Mechanisms to move and steer chromosomesR01GM124042 · NIGMS · UNIVERSITY OF VIRGINIA · PI STUKENBERG, P. TODD · 2018 to 2021
$1.7M
NCI NIH HHS P30 CA044579NIGMS NIH HHS R01 GM124042NIGMS NIH HHS R35 GM148181NIGMS NIH HHS T32 GM008136NINDS NIH HHS R01 NS036267
6 · The paper itself

Abstract

Dynein inactivates the spindle assembly checkpoint (SAC) by transporting checkpoint proteins away from kinetochores toward spindle poles in a process known as "stripping." We find that inhibition of Aurora A kinase, which is localized to spindle poles, enables the accumulation of the spindle checkpoint activator Mad1 at poles where it is normally absent. Aurora kinases phosphorylate the dynein activator NudE neurodevelopment protein 1 like 1 (Ndel1) on Ser285 and Mad1 accumulates at poles when Ndel1 is replaced by a nonphosphorylatable mutant in human cells. The pole focusing protein NuMA, transported to poles by dynein, also accumulates at poles in cells harboring a mutant Ndel1. Phosphorylation of Ndel1 on Ser285 is required for robust spindle checkpoint activity and regulates the poles of asters in

Indexed as

DyneinsSpindle ApparatusAurora Kinase ACarrier ProteinsCell Cycle ProteinsHumansKinetochoresMicrotubulesSpindle PolesAurora Kinase ACarrier ProteinsCell Cycle ProteinsDyneinsNDEL1 protein, human

Identifiers

PMID36350697
PMCPMC9816647
OpenAlexW4308639323

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.