Evidence map›Paper›PMID 36350555›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Use of Deubiquitinase Fusion Proteins to Characterize Endocytic Trafficking in Yeast.

Nathaniel L Hepowit, Aeva S Denise, Jason A MacGurn

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Nathaniel L HepowitDepartment of Cell and Developmental Biology, Vanderbilt University, Nashville, TN, USA.
Aeva S DeniseDepartment of Cell and Developmental Biology, Vanderbilt University, Nashville, TN, USA.
Jason A MacGurnDepartment of Cell and Developmental Biology, Vanderbilt University, Nashville, TN, USA. jason.a.macgurn@vanderbilt.edu.
Vanderbilt University · US

Funding

Deciphering the ubiquitin code in stress signaling and membrane traffickingR35GM144112 · NIGMS · VANDERBILT UNIVERSITY · PI Jason A MacGurn · 2022 to 2026
$2.2M
NIGMS NIH HHS R35 GM144112
6 · The paper itself

Abstract

Ubiquitin modification is known to regulate endocytic trafficking of many different types of cargo in eukaryotic cells, but it can be challenging to determine what role, if any, ubiquitin plays in the trafficking of a novel or uncharacterized endocytic cargo. Here, we describe a useful approach that leverages fusion to deubiquitinase (DUB) catalytic domains to explore the role ubiquitin plays in endocytic trafficking. This approach can be applied to the analysis of many different endocytic cargos in different cell types, and it can also be used to study linkage specificity in endocytic trafficking. Several different trafficking assays are described to illustrate the broad utility of this "DUB fusion" approach.

Indexed as

Saccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsDeubiquitinating EnzymesEndocytosisUbiquitinDeubiquitinating EnzymesSaccharomyces cerevisiae ProteinsUbiquitinCan1DUB fusionEndocytosisFlow cytometryFluorescence microscopyMup1Spot plate dilution assay

Identifiers

PMID36350555
OpenAlexW4308750618

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.