Evidence map›Paper›PMID 36350552›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Identification of Deubiquitinase Substrates in Saccharomyces cerevisiae by Systematic Overexpression.

Heather E Arsenault, Jennifer A Benanti

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Heather E ArsenaultDepartment of Molecular, Cell and Cancer Biology, University of Massachusetts Chan Medical School, Worcester, MA, USA.
Jennifer A BenantiDepartment of Molecular, Cell and Cancer Biology, University of Massachusetts Chan Medical School, Worcester, MA, USA. jennifer.benanti@umassmed.edu.

Funding

Molecular Mechanisms of Cell Cycle ControlR35GM136280 · NIGMS · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI Jennifer A Benanti · 2020 to 2026
$4.8M
NIGMS NIH HHS R35 GM136280
6 · The paper itself

Abstract

A significant hurdle to understanding the functions of deubiquitinases (DUBs) is the identification of their in vivo substrates. Substrate identification can be difficult for two reasons. First, many proteins that are degraded by the ubiquitin-proteasome system are expressed at relatively low levels in the cell, and second, redundancy between DUBs complicates loss of function screening approaches. Here, we describe a systematic overexpression approach that takes advantage of genome-wide resources available in S. cerevisiae to overcome these challenges and identify DUB substrates in cells.

Indexed as

Saccharomyces cerevisiaeUbiquitinDeubiquitinating EnzymesProteasome Endopeptidase ComplexDeubiquitinating EnzymesProteasome Endopeptidase ComplexUbiquitinCycloheximide chaseDeubiquitinaseOverexpressionWestern blottingYeast

Identifiers

PMID36350552

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.