Evidence map›Paper›PMID 36350540›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Synthetic Diubiquitin Fluorogenic Substrates to Study DUBs.

Max S Kloet, Gerbrand J van der Heden van Noort

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Max S KloetOncode Institute and Department of Cell and Chemical Biology, Leiden University Medical Centre, Leiden, The Netherlands.
Gerbrand J van der Heden van NoortOncode Institute and Department of Cell and Chemical Biology, Leiden University Medical Centre, Leiden, The Netherlands. gvanderheden@lumc.nl.

Funding

Deciphering the ubiquitin code in stress signaling and membrane traffickingR35GM144112 · NIGMS · VANDERBILT UNIVERSITY · PI Jason A MacGurn · 2022 to 2026
$2.2M
NIGMS NIH HHS R35 GM144112
6 · The paper itself

Abstract

Development of (semi-)synthetic methods to prepare ubiquitin (Ub)-based reagents has proven to be helpful in the classification of deubiquitinating proteases (DUBs). To study DUB selectivity for one or more of the eight possible poly-Ub chains, fluorogenic assay reagents have been reported relying on the appearance of a fluorescent signal upon DUB-mediated cleavage of the reagent. In this protocol, we describe the use of such an assay to profile the selectivity of TRABID, a member of the OTU family of DUBs.

Indexed as

Fluorescent DyesUbiquitinsEndopeptidasesPeptide HydrolasesUbiquitinUbiquitinationEndopeptidasesFluorescent DyesPeptide HydrolasesUbiquitinUbiquitinsActivity assayDeubiquitinating proteaseFluorescenceFluorogenic substrateTRABID

Identifiers

PMID36350540

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.