Evidence map›Paper›PMID 36349224›Full record

ArticleRSC chemical biology2022

Methyltetrazine as a small live-cell compatible bioorthogonal handle for imaging enzyme activities

Diana Torres-García, Merel A T van de Plassche, Emma van Boven, Tyrza van Leeuwen, Mirjam G J Groenewold, Alexi J C Sarris, Luuk Klein, Herman S Overkleeft, Sander I van Kasteren

Abstract read
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In one paragraph

Article in RSC chemical biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Diana Torres-GarcíaLeiden Institute of Chemistry and The Institute for Chemical Immunology, Leiden University Einsteinweg 55 2333 CC Leiden The Netherlands s.i.van.kasteren@chem.leidenuniv.nl h.s.overkleeft@lic.leidenuniv.nl.
Merel A T van de PlasscheLeiden Institute of Chemistry and The Institute for Chemical Immunology, Leiden University Einsteinweg 55 2333 CC Leiden The Netherlands s.i.van.kasteren@chem.leidenuniv.nl h.s.overkleeft@lic.leidenuniv.nl.
Emma van BovenLeiden Institute of Chemistry and The Institute for Chemical Immunology, Leiden University Einsteinweg 55 2333 CC Leiden The Netherlands s.i.van.kasteren@chem.leidenuniv.nl h.s.overkleeft@lic.leidenuniv.nl.
Tyrza van LeeuwenLeiden Institute of Chemistry and The Institute for Chemical Immunology, Leiden University Einsteinweg 55 2333 CC Leiden The Netherlands s.i.van.kasteren@chem.leidenuniv.nl h.s.overkleeft@lic.leidenuniv.nl.
Mirjam G J GroenewoldLeiden Institute of Chemistry and The Institute for Chemical Immunology, Leiden University Einsteinweg 55 2333 CC Leiden The Netherlands s.i.van.kasteren@chem.leidenuniv.nl h.s.overkleeft@lic.leidenuniv.nl.
Alexi J C SarrisLeiden Institute of Chemistry and The Institute for Chemical Immunology, Leiden University Einsteinweg 55 2333 CC Leiden The Netherlands s.i.van.kasteren@chem.leidenuniv.nl h.s.overkleeft@lic.leidenuniv.nl.
Luuk KleinLeiden Institute of Chemistry and The Institute for Chemical Immunology, Leiden University Einsteinweg 55 2333 CC Leiden The Netherlands s.i.van.kasteren@chem.leidenuniv.nl h.s.overkleeft@lic.leidenuniv.nl.
Herman S OverkleeftLeiden Institute of Chemistry and The Institute for Chemical Immunology, Leiden University Einsteinweg 55 2333 CC Leiden The Netherlands s.i.van.kasteren@chem.leidenuniv.nl h.s.overkleeft@lic.leidenuniv.nl.ORCID https://orcid.org/0000-0001-6976-7005
Sander I van KasterenLeiden Institute of Chemistry and The Institute for Chemical Immunology, Leiden University Einsteinweg 55 2333 CC Leiden The Netherlands s.i.van.kasteren@chem.leidenuniv.nl h.s.overkleeft@lic.leidenuniv.nl.ORCID https://orcid.org/0000-0003-3733-818X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bioorthogonal chemistry combines well with activity-based protein profiling, as it allows for the introduction of detection tags without significantly influencing the physiochemical and biological functions of the probe. In this work, we introduced methyltetrazinylalanine (MeTz-Ala), a close mimic of phenylalanine, into a dipeptide fluoromethylketone cysteine protease inhibitor. Following covalent and irreversible inhibition, the tetrazine allows vizualisation of the captured cathepsin activity by means of inverse electron demand Diels Alder ligation in cell lysates and live cells, demonstrating that tetrazines can be used as live cell compatible, minimal bioorthogonal tags in activity-based protein profiling.

Identifiers

PMID36349224
PMCPMC9627743

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.