Analytical Method for Experimental Validation of Computer-Designed Antibody.

Aki Tanabe, Kouhei Tsumoto

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it

0cells of the map it votes in

2citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

Accelerating antibody discovery and optimization with high-throughput experimentation and machine learning.Journal of biomedical science · 2025
Review
FASTIA: A rapid and accessible platform for protein variant interaction analysis demonstrated with a single-domain antibody.Protein science : a publication of the Protein Society · 2025
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Aki TanabeDepartment of Bioengineering, School of Engineering, The University of Tokyo, Tokyo, Japan.

Kouhei TsumotoDepartment of Bioengineering, School of Engineering, The University of Tokyo, Tokyo, Japan. tsumoto@bioeng.t.u-tokyo.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In the computational design of antibodies, the interaction analysis between target antigen and antibody is an essential process to obtain feedback for validation and optimization of the design. Kinetic and thermodynamic parameters as well as binding affinity (K

Indexed as

AntibodiesAntigensComputersEnzyme-Linked Immunosorbent AssayKineticsAntibodiesAntigensAffinityBiolayer interferometry (BLI)ELISAExperimental validationFlow cytometry (FCM)Fluorescence polarization (FP)Interaction analysis of antigen–antibodyKinetic parametersMicroscale thermophoresis (MST)Thermodynamic parameters

Identifiers

PMID36346606

DOI10.1021/jp410640z

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.