ArticleACS applied materials & interfaces2022
Di-Arginine Additives for Dissociation of Gold Nanoparticle Aggregates: A Matrix-Insensitive Approach with Applications in Protease Detection.
Article in ACS applied materials & interfaces, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 21 citations in OpenAlex.
- Reactive Additive-Induced Joining of Apolar Thiol-Coated Gold Nanoparticle Cores at Low Temperatures.Langmuir : the ACS journal of surfaces and colloids · 2026Article
- Ligand Desorption and Surface Oxidation Drive Nanoparticle Coalescence in Diffusion-Limited Aggregation.Journal of the American Chemical Society · 2025Article
- Mechanism of Cationic Peptide-Induced Assembly of Gold Nanoparticles: Modulation of Electrostatic Repulsion.Aggregate (Hoboken, N.J.) · 2025Article
- Activatable Photoacoustic Probe for Imaging Infection: Gold Nanorod Dissociation In Vivo Reports Bacterial Protease Activity.ACS nano · 2025Article
- Mechanism of hierarchical plasmonic biomaterials engineered through peptide-directed self-assembly.Aggregate (Hoboken, N.J.) · 2025Article
- Colorimetric sensing for translational applications: from colorants to mechanisms.Chemical Society reviews · 2024Review
- Rapid colorimetric sensing of chlorpromazine HCl antipsychotic throughRSC advances · 2024Article
- Goldilocks Energy Minimum: Peptide-Based Reversible Aggregation and Biosensing.ACS applied materials & interfaces · 2023Article
- A Protease-Responsive Polymer/Peptide Conjugate and Reversible Assembly of Silver Clusters for the Detection ofACS nano · 2023Article
- An approach to zwitterionic peptide design for colorimetric detection of the Southampton norovirus SV3CP protease.The Analyst · 2023Article
- Empirical Optimization of Peptide Sequence and Nanoparticle Colloidal Stability: The Impact of Surface Ligands and Implications for Colorimetric Sensing.ACS applied materials & interfaces · 2023Article
- A fluoride-induced aggregation test to quickly assess the efficiency of ligand exchange procedures from citrate capped AuNPs.Colloids and surfaces. A, Physicochemical and engineering aspects · 2023Article
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
We report the reversible aggregation of gold nanoparticles (AuNPs) assemblies via a di-arginine peptide additive and thiolated PEGs (HS-PEGs). The AuNPs were first aggregated by attractive forces between the citrate-capped surface and the arginine side chains. We found that the HS-PEG thiol group has a higher affinity for the AuNP surface, thus leading to redispersion and colloidal stability. In turn, there was a robust and obvious color change due to on/off plasmonic coupling. The assemblies' dissociation was directly related to the HS-PEG structural properties such as their size or charge. As an example, HS-PEGs with a molecular weight below 1 kDa could dissociate 100% of the assemblies and restore the exact optical properties of the initial AuNP suspension (prior to the assembly). Surprisingly, the dissociation capacity of HS-PEGs was not affected by the composition of the operating medium and could be performed in complex matrices such as plasma, saliva, bile, urine, cell lysates, or even seawater. The high affinity of thiols for the gold surface encompasses by far the one of endogenous molecules and is thus favored. Moreover, starting with AuNPs already aggregated ensured the absence of a background signal as the dissociation of the assemblies was far from spontaneous. Remarkably, it was possible to dry the AuNP assemblies and solubilize them back with HS-PEGs, improving the colorimetric signal generation. We used this system for protease sensing in biological fluids. Trypsin was chosen as the model enzyme, and highly positively charged peptides were conjugated to HS-PEG molecules as cleavage substrates. The increase of positive charge of the HS-PEG-peptide conjugate quenched the dissociation capacity of the HS-PEG molecules, which could only be restored by the proteolytic cleavage. Picomolar limit of detection was obtained as well as the detection in saliva or urine.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.