Evidence map›Paper›PMID 36345183›Full record

ArticleJoint diseases and related surgery2022

The effects of thymoquinone on steroid-induced femoral head osteonecrosis: An experimental study in rats.

Mustafa Fatih Dasci, Elif Yaprak Sarac, Asiye Gok Yurttas, Tolgahan Atci, Muhammed Uslu, Abdurrahman Acar, Mehmet Akif Gulec, Ender Alagoz

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Joint diseases and related surgery, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed, 1 pooled it
1.3field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 1 synthesis or guideline pooled it, 7 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 5 institutions in 3 countries.

Mustafa Fatih Dasci
Elif Yaprak Sarac
Asiye Gok Yurttas
Tolgahan Atci
Muhammed Uslu
Abdurrahman Acar
Mehmet Akif Gulec
Ender AlagozSBÜ Bağcılar Eğitim ve Araştırma Hastanesi Ortopedi ve Travmatoloji Kliniği, 34200 Bağcılar, İstanbul, Türkiye.
Department of Health and Social Care · GBİstanbul Eğitim ve Araştırma Hastanesi · TRIstanbul Health and Technology UniversityIstanbul Technical University · TRSağlık Bilimleri Üniversitesi · TR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesIn this study, we aimed to evaluate the antioxidant, anti-apoptotic, osteoblastic and hypolipidemic effects of thymoquinone (TQ) treatment on the steroid-induced osteonecrosis of femoral head (ONFH) model in rats. MATERIALS AND

methodsA total of 24 rats were randomly divided into four groups: the control group administered saline; the TQ group administered 10 mg/kg/day TQ orally; lipopolysaccharide/methylprednisolone (LPS/MPS) group administered 20 µg/kg intraperitoneally LPS and 40 mg/kg intramuscularly MPS to establish ONFH model; and the LPS/ MPS+TQ group administered both LPS/MPS and, then, TQ once daily for four weeks. All rats were sacrificed after intracardiac blood collection and their right femurs were removed.

resultsMicro-computed tomography showed a higher bone mineral density and lower porosity, Tr. Sp and Tr. Sep data were detected in the LPS/MPS+TQ group. In histopathology, osteonecrosis increased significantly in the LPS/MPS group and osteonecrosis decreased in the LPS/MPS+TQ group compared to the LPS/MPS group (p=0.0077). Histomorphometric examination revealed that the percentage of BV/TV in the LPS/MPS group was significantly lower compared to control and other groups (p<0.01 and p<0.05, respectively), while it reached normal rates in the LPS/MPS+TQ group. Immunohistochemically, antioxidant, anti-apoptotic, and angiogenesis indicators (8-hydroxy-20- deoxyguanosine [8-OHdG], malondialdehyde [MDA], B-cell lymphoma [Bcl-2], caspase-3, vascular endothelial growth factor [VEGF]) were significantly improved in tissue and serum with TQ. Furthermore, TQ significantly reduced low- and high-density lipoprotein cholesterol ratio and carboxy-terminal type 1 collagen crosslink (CTX) in serum.

conclusionVascular and hematopoietic cell damages that occur due to steroid-induced deoxyribonucleic acid (DNA) oxidative and lipid peroxidative damages in an ONFH model can be successfully ameliorated by TQ administration. This antioxidant and anti-apoptotic effects of TQ may be a promising treatment option for early stage of osteonecrosis.

Indexed as

Femur HeadFemur Head NecrosisAnimalsAntioxidantsBenzoquinonesDisease Models, AnimalLipopolysaccharidesMethylprednisoloneRatsSteroidsVascular Endothelial Growth Factor AX-Ray MicrotomographyAntioxidantsBenzoquinonesLipopolysaccharidesMethylprednisoloneSteroidsthymoquinoneVascular Endothelial Growth Factor A

Identifiers

PMID36345183
PMCPMC9647667
OpenAlexW4308004537

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.