ArticleNature biotechnology2023
MINSTED nanoscopy enters the Ångström localization range.
Article in Nature biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 86 citations in OpenAlex.
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- Advances, Challenges, and Opportunities in Electrochemiluminescence Bioimaging.Chemical & biomedical imaging · 2026Review
- Mirror-enhanced 4Pi-SMLM with one objective enables isotropic nanoscale imaging.Nature biotechnology · 2026Article
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- Topology-driven energy transfer networks for upconversion stimulated emission depletion microscopy.Light, science & applications · 2025Article
- Cellular optical imaging techniques: a dynamic advancing frontier.Science China. Life sciences · 2025Review
- Advancing Single-Molecule Biophysics: Next-Generation Organic Fluorophores with Tailored Labeling Strategies.Chemical & biomedical imaging · 2025Review
- Super-resolution microscopy for structural biology.Nature methods · 2025Review
- Open-source 3D active sample stabilization for fluorescence microscopy.Biophysical reports · 2025Article
- Image-based 3D active sample stabilization on the nanometer scale for optical microscopy.Biophysical reports · 2025Article
- Multiparametric super-resolution optoacoustic imaging with ICG-tagged microbubbles.Materials today. Bio · 2025Article
- Bridge to a brighter future in fluorescence imaging.Nature methods · 2025Article
- MINFLUX Nanoscopy: A "Brilliant" Technique Promising Major Breakthrough.Microscopy research and technique · 2025Review
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- Multidimensional multiplexing metalens for STED microscopy.Science advances · 2025Article
- Resolving the Orientations of and Angular Separation Between a Pair of Dipole Emitters.Physical review letters · 2025Article
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9 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Super-resolution techniques have achieved localization precisions in the nanometer regime. Here we report all-optical, room temperature localization of fluorophores with precision in the Ångström range. We built on the concept of MINSTED nanoscopy where precision is increased by encircling the fluorophore with the low-intensity central region of a stimulated emission depletion (STED) donut beam while constantly increasing the absolute donut power. By blue-shifting the STED beam and separating fluorophores by on/off switching, individual fluorophores bound to a DNA strand are localized with σ = 4.7 Å, corresponding to a fraction of the fluorophore size, with only 2,000 detected photons. MINSTED fluorescence nanoscopy with single-digit nanometer resolution is exemplified by imaging nuclear pore complexes and the distribution of nuclear lamin in mammalian cells labeled by transient DNA hybridization. Because our experiments yield a localization precision σ = 2.3 Å, estimated for 10,000 detected photons, we anticipate that MINSTED will open up new areas of application in the study of macromolecular complexes in cells.
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