ArticleScientific reports2022
Anti-biofilm activity of caffeine against uropathogenic E. coli is mediated by curli biogenesis.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
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- Authenticity Assessment of Five Monofloral Honeys Based on Phytochemical Profiles.Foods (Basel, Switzerland) · 2026Article
- Alkaloid-based nanodrug delivery systems: An emerging strategy against drug-resistant bacterial wound infections.International journal of pharmaceutics: X · 2026Review
- Antimicrobial and Cytoprotective Effects of Tea Extracts AgainstAntibiotics (Basel, Switzerland) · 2025Article
- Antibiofilm Activities of Halogenated Pyrimidines Against EnterohemorrhagicInternational journal of molecular sciences · 2025Article
- Discovery of New Natural Phytocompounds: The Modern Tools to Fight Against Traditional Bacterial Pathogens.Current pharmaceutical biotechnology · 2025Review
- Valorization of Coffee Cherry By-Products Through Fermentation by Human Intestinal Lactobacilli in Functional Fermented Milk Beverages.Foods (Basel, Switzerland) · 2024Article
- Exploring Naturally Tailored Bacterial Outer Membrane Vesicles for Selective Bacteriostatic Implant Coatings.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Caffeine Protects Keratinocytes fromInternational journal of molecular sciences · 2024Article
- Biogenic amine tryptamine in human vaginal probiotic isolates mediates matrix inhibition and thwarts uropathogenic E. coli biofilm.Scientific reports · 2024Article
- Natural Antimicrobial Mixtures Disrupt Attachment and Survival ofFoods (Basel, Switzerland) · 2023Article
- Restoring Ampicillin Sensitivity in Multidrug-ResistantJournal of microbiology and biotechnology · 2023Article
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Corrections and comments
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biofilms are assemblages of sessile microorganisms that form an extracellular matrix around themselves and mediate attachment to surfaces. The major component of the extracellular matrix of Uropathogenic E. coli and other Enterobacteriaceae are curli fibers, making biofilms robust and resistant to antimicrobials. It is therefore imperative to screen antibiofilm compounds that can impair biofilm formation. In the present study, we investigated the curli-dependent antibiofilm activity of caffeine against UPEC strain CFT073 and commensal strain E. coli K-12MG1655.Caffeine significantly reduced the biofilm formation of both UPEC and E. coli K-12 by 86.58% and 91.80% respectively at 48 mM caffeine as determined by Crystal Violet assay. These results were further confirmed by fluorescence microscopy and Scanning Electron Microscope (SEM). Caffeine significantly reduced the cytotoxicity and survivability of UPEC. Molecular docking analysis revealed a strong interaction between caffeine and curli regulator protein (Csg D) of E. coli. The qRT-PCR data also showed significant downregulation in the expression of CsgBA and the CsgDEFG operon at both 24 mM and 48 mM caffeine. The findings revealed that caffeine could inhibit E. coli biofilm formation by regulating curli assembly and thus may be used as an alternative therapeutic strategy for the treatment of chronic E. coli biofilm-related infections.
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