ArticlePlanta2022
CRISPR/Cas-mediated knockdown of vacuolar invertase gene expression lowers the cold-induced sweetening in potatoes.
Article in Planta, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
- Molecular Targets and Trait Innovation in CRISPR-Edited Vegetable Crops: An Up-to-Date Review (2020-2026).Plants (Basel, Switzerland) · 2026Review
- Advances in CRISPR Plant Applications.International journal of molecular sciences · 2026Review
- Research progress on cold tolerance key genes and mechanisms in four major northern China crops.Frontiers in plant science · 2026Review
- Integrated transcriptomic and metabolomic analyses reveal the molecular mechanisms of sugar accumulation and yield trade-off in sugar beet.Frontiers in plant science · 2026Article
- A novel recombinant CRISPR/Cas9 vector system for genome editing in plants.Transgenic research · 2025Article
- CRISPR-Cas Gene Editing Technology in Potato.International journal of molecular sciences · 2025Review
- Molecular Mechanisms Underlying Defense Responses of Potato (Plants (Basel, Switzerland) · 2025Review
- CRISPR mediated gene editing for economically important traits in horticultural crops: progress and prospects.Transgenic research · 2025Review
- CRISPR/Cas9-Mediated Development of Potato Varieties with Long-Term Cold Storage and Bruising Resistance.Biology · 2025Article
- Accelerating crop improvement via integration of transcriptome-based network biology and genome editing.Planta · 2025Review
- Potato: from functional genomics to genetic improvement.Molecular horticulture · 2024Review
- Identification, characterisation and expression analysis of peanut sugar invertase genes reveal their vital roles in response to abiotic stress.Plant cell reports · 2024Article
- Recent advances of CRISPR-based genome editing for enhancing staple crops.Frontiers in plant science · 2024Review
- Genome-wide identification and analysis of the invertase gene family in tobacco (Frontiers in plant science · 2023Article
- Amelioration of cold-induced sweetening in potato by RNAi mediated silencing ofFrontiers in plant science · 2023Article
- Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MAIN
conclusionVInv gene editing in potato using CRISPR/Cas9 resulted in knockdown of expression and a lower VInv enzymatic activity resulting in a decrease in post-harvest cold-storage sugars formation and sweetening in potatoes. CRISPR-Cas9-mediated knockdown of vacuolar invertase (VInv) gene was carried out using two sgRNAs in local cultivar of potato plants. The transformation efficiency of potatoes was found to be 11.7%. The primary transformants were screened through PCR, Sanger sequencing, digital PCR, and ELISA. The overall editing efficacy was determined to be 25.6% as per TIDE analysis. The amplicon sequencing data showed maximum indel frequency for potato plant T12 (14.3%) resulting in 6.2% gene knockout and 6% frame shift. While for plant B4, the maximum indel frequency of 2.0% was found which resulted in 4.4% knockout and 4% frameshift as analyzed by Geneious. The qRT-PCR data revealed that mRNA expression of VInv gene was reduced 90-99-fold in edited potato plants when compared to the non-edited control potato plant. Following cold storage, chips analysis of potatoes proved B4 and T12 as best lines. Reducing sugars' analysis by titration method determined fivefold reduction in percentage of reducing sugars in tubers of B4 transgenic lines as compared to the control. Physiologically genome-edited potatoes behaved like their conventional counterpart. This is first successful report of knockdown of potato VInv gene in Pakistan that addressed cold-induced sweetening resulting in minimum accumulation of reducing sugars in genome edited tubers.
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