Evidence map›Paper›PMID 36341746›Full record

ArticleBiomaterials advances2022

3D printing a biocompatible elastomer for modeling muscle regeneration after volumetric muscle loss.

Wisarut Kiratitanaporn, David B Berry, Anusorn Mudla, Trevor Fried, Alison Lao, Claire Yu, Nan Hao, Samuel R Ward, Shaochen Chen

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials advances, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.5field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 18 citations in OpenAlex.

  1. Review
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  3. Article
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  5. Review
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  7. Light-based vat-polymerization bioprinting.Nature reviews. Methods primers · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Wisarut KiratitanapornDepartment of Bioengineering, University of California, 9500 Gilman Dr. San Diego, La Jolla, CA 92093, USA. Electronic address: wkiratit@eng.ucsd.edu.
David B BerryDepartment of NanoEngineering, University of California, 9500 Gilman Dr. San Diego, La Jolla, CA 92093, USA; Department of Orthopaedic Surgery, University of California, 9500 Gilman Dr. San Diego, La Jolla, CA 92093, USA. Electronic address: dbberry@ucsd.edu.
Anusorn MudlaDepartment of Section of Molecular Biology, Division of Biological Sciences, University of California, 9500 Gilman Dr. San Diego, La Jolla, CA, 92093, USA. Electronic address: amudla@ucsd.edu.
Trevor FriedDepartment of Bioengineering, University of California, 9500 Gilman Dr. San Diego, La Jolla, CA 92093, USA. Electronic address: tfried@ucsd.edu.
Alison LaoDepartment of NanoEngineering, University of California, 9500 Gilman Dr. San Diego, La Jolla, CA 92093, USA. Electronic address: a5lao@ucsd.edu.
Claire YuDepartment of NanoEngineering, University of California, 9500 Gilman Dr. San Diego, La Jolla, CA 92093, USA. Electronic address: hyy039@eng.ucsd.edu.
Nan HaoDepartment of Section of Molecular Biology, Division of Biological Sciences, University of California, 9500 Gilman Dr. San Diego, La Jolla, CA, 92093, USA. Electronic address: nhao@ucsd.edu.
Samuel R WardDepartment of Bioengineering, University of California, 9500 Gilman Dr. San Diego, La Jolla, CA 92093, USA; Department of Orthopaedic Surgery, University of California, 9500 Gilman Dr. San Diego, La Jolla, CA 92093, USA; Department of Radiology, University of California, 9500 Gilman Dr. San Diego, La Jolla, CA 92093, USA. Electronic address: s1ward@health.ucsd.edu.
Shaochen ChenDepartment of Bioengineering, University of California, 9500 Gilman Dr. San Diego, La Jolla, CA 92093, USA; Department of NanoEngineering, University of California, 9500 Gilman Dr. San Diego, La Jolla, CA 92093, USA. Electronic address: chen168@eng.ucsd.edu.
University of California San Diego · US

Funding

U of Calif, San Diego Neuroscience Microscopy ImagingP30NS047101 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI GLEESON, JOSEPH G, ZHENG, BINHAI · 2003 to 2022
$9.0M
Non Invasive Measurements of Muscle Microstructures Assessed by Diffusion Tensor ImagingR01AR070830 · NIAMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI David Barnes Berry, LAWRENCE R FRANK · 2017 to 2026
$4.0M
Dynamically compartmentalized control of gene expression by messenger ribonucleoprotein granulesR01GM111458 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI HAO, NAN · 2014 to 2022
$3.4M
3D Printing of Precision Scaffolds for Volumetric Muscle Tissue RegenerationR21AR074763 · NIAMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CHEN, SHAOCHEN · 2019 to 2020
$355k
NIAMS NIH HHS R01 AR070830NIAMS NIH HHS R21 AR074763NIGMS NIH HHS R01 GM111458NINDS NIH HHS P30 NS047101
6 · The paper itself

Abstract

Volumetric muscle loss (VML) injuries due to trauma, tumor ablation, or other degenerative muscle diseases are debilitating and currently have limited options for self-repair. Advancements in 3D printing allow for the rapid fabrication of biocompatible scaffolds with designer patterns. However, the materials chosen are often stiff or brittle, which is not optimal for muscle tissue engineering. This study utilized a photopolymerizable biocompatible elastomer - poly (glycerol sebacate) acrylate (PGSA) - to develop an in vitro model of muscle regeneration and proliferation into an acellular scaffold after VML injury. Mechanical properties of the scaffold were tuned by controlling light intensity during the 3D printing process to match the specific tension of skeletal muscle. The effect of both geometric (channel sizes between 300 and 600 μm) and biologic (decellularized muscle extracellular matrix (dECM)) cues on muscle progenitor cell infiltration, proliferation, organization, and maturation was evaluated in vitro using a near-infrared fluorescent protein (iRFP) transfected cell line to assess cells in the 3D scaffold. Larger channel sizes and dECM coating were found to enhance cell proliferation and maturation, while no discernable effect on cell alignment was observed. In addition, a pilot experiment was carried out to evaluate the regenerative capacity of this scaffold in vivo after a VML injury. Overall, this platform demonstrates a simple model to study muscle progenitor recruitment and differentiation into acellular scaffolds after VML repair.

Indexed as

ElastomersMuscular DiseasesDecanoatesGlycerolHumansMuscle, SkeletalPolymersPrinting, Three-DimensionalRegenerationTissue EngineeringDecanoatesElastomersGlycerolpoly(glycerol-sebacate)Polymers3D printingDLPPoly glycerol sebacate acrylateSkeletal muscleVolumetric muscle loss

Identifiers

PMID36341746
PMCPMC12045644
OpenAlexW4307297270

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.