ArticleFrontiers in cellular and infection microbiology2022
Stable reconstructed human gingiva-microbe interaction model: Differential response to commensals and pathogens.
Article in Frontiers in cellular and infection microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 9 citations in OpenAlex.
- November in focus in HCB.Histochemistry and cell biology · 2025Article
- Mechanical-enzymatic isolation and characterization of primary human gingival epithelial cells for reproducible in vitro oral mucosa models.Histochemistry and cell biology · 2025Article
- An in vitro model demonstrating homeostatic interactions between reconstructed human gingiva and a saliva-derived multispecies biofilm.Microbiome · 2025Article
- Advances in modeling periodontal host-microbe interactions: insights from organotypic and organ-on-chip systems.Lab on a chip · 2025Review
- Article
- The oral-gut microbiome axis in health and disease.Nature reviews. Microbiology · 2024Review
- Illuminating the oral microbiome: cellular microbiology.FEMS microbiology reviews · 2023Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: To investigate human oral health and disease, models are required which represent the interactions between the oral mucosa and microbiome. Our aim was to develop an organotypic model which maintains viability of both host and microbes for an extended period of time. Methods: Reconstructed Human Gingiva (RHG) were cultured air-lifted with or without penicillin-streptomycin (PS) and topically exposed to Results: The low concentration of topically applied agar did not influence RHG viability. Topically applied bacteria in agar remained localized and viable for 72 hours and did not spill over to infect RHG culture medium. PS in RHG culture medium killed topically applied bacteria. Co-culture with living bacteria did not influence RHG viability (Ki67 expression, MTT assay) or histology (epithelium differentiation, Keratin10 expression). RHG exposed to Conclusion: This technical advance enables living human oral host-microbe interactions to be investigated during a 72-hour period and shows differences in innate immunology triggered by
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