ArticleFrontiers in cellular and infection microbiology2022
Development and evaluation of a rapid and sensitive multienzyme isothermal rapid amplification with a lateral flow dipstick assay for detection of
Article in Frontiers in cellular and infection microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Rapid On-Site Detection ofFoods (Basel, Switzerland) · 2026Article
- Multiple cross displacement amplification coupled with a lateral flow biosensor for ultra-rapid and highly sensitive detection ofMicrobiology spectrum · 2026Article
- Duplex MIRA assays for simultaneous detection of novel goose astrovirus and goose circovirus.BMC veterinary research · 2026Article
- Selection of ssDNA aptamers targeting the serine protease SPSFQ of Acinetobacter baumannii and development of an electrochemical impedance spectroscopy-based ultrasensitive SPSFQ biosensor.Mikrochimica acta · 2026Article
- Establishment of duplex multi-enzyme isothermal rapid amplification detection method for bovine astrovirus and norovirus.Frontiers in veterinary science · 2026Article
- A rapid visual detection method for indel polymorphisms in the bovine PRNP gene based on a duplex MIRA-LFD assay.Frontiers in veterinary science · 2026Article
- Article
- Development and evaluation of a real-time multienzyme isothermal rapid amplification assay for rapid detection of Streptococcus pneumoniae.Scientific reports · 2024Article
- Simple and field-adapted species identification of biological specimens combining multiplex multienzyme isothermal rapid amplification, lateral flow dipsticks, and universal primers for initial rapid screening without standard PCR laboratory.International journal of legal medicine · 2024Article
- Development of Multienzyme Isothermal Rapid Amplification (MIRA) Combined with Lateral-Flow Dipstick (LFD) Assay to Detect Species-SpecificPathogens (Basel, Switzerland) · 2024Article
- Recombinase polymerase amplification combined with lateral flow biosensor for rapid visual detection ofFrontiers in veterinary science · 2024Article
- Review
- Rapid and Sensitive Detection ofInternational journal of molecular sciences · 2023Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Purpose: This study aimed to establish the multienzyme isothermal rapid amplification with a lateral flow dipstick (MIRA-LFD) assay and evaluate its performance in detection of Methods: The study was divided into two stages: a pilot study to establish the methodology and a clinical validation study to evaluate its performance. In the first step, we designed primers specific to detect Results: The developed MIRA-LFD assay displayed a detection limit of 6 CFU/mL for detecting Conclusions: This study demonstrates that the established MIRA-LFD assay is time-saving, more effective and sensitive, which may become a powerful tool for rapid and reliable diagnosis of bloodstream infection caused by
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