Evidence map›Paper›PMID 36338819›Full record

ArticleJDS communications2022

Culture of endometrial epithelial cells collected by a cytological brush in vivo.

Cecilia Constantino Rocha, Felipe Alves Correa Silva, Thiago Martins, Marcela G Marrero, John J Bromfield, John Driver, Thomas Hansen, Lilian Oliveira, Mario Binelli

Open access · goldAbstract read
In one paragraph

Article in JDS communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.4field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 13 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Cecilia Constantino RochaDepartment of Animal Sciences, University of Florida, Gainesville 32611.
Felipe Alves Correa SilvaDepartment of Animal Sciences, University of Florida, Gainesville 32611.
Thiago MartinsDepartment of Animal Sciences, University of Florida, Gainesville 32611.
Marcela G MarreroDepartment of Animal Sciences, University of Florida, Gainesville 32611.
John J BromfieldDepartment of Animal Sciences, University of Florida, Gainesville 32611.
John DriverDepartment of Animal Sciences, University of Florida, Gainesville 32611.
Thomas HansenDepartment of Biomedical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80521.
Lilian OliveiraDepartment of Pathology, College of Veterinary Medicine, University of Georgia, Athens 30602.
Mario BinelliDepartment of Animal Sciences, University of Florida, Gainesville 32611.
University of Florida · USColorado State University · USUniversity of Georgia · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In cattle, mechanistic studies of endometrial function rely on cell lines or primary culture of cells harvested postmortem. Understanding the endometrial physiology in dairy cows is essential, because approximately 50% of pregnancies are lost in the first 3 wk of gestation for unknown reasons. The objective was to validate an in vivo, minimally invasive, and estrous cycle stage-specific method to obtain endometrial luminal epithelial cells for culture. The uterine body of 26 cows was sampled using a cytology brush (cytobrush) 4 d after estrus. The viability of cells was measured by flow cytometry (80% live cells) and epithelial identity was determined by anti-vimentin and anti-cytokeratin immunofluorescence and quantitative PCR for

Identifiers

PMID36338819
PMCPMC9623749
OpenAlexW4220660221

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.