ArticleFrontiers in neural circuits2022
Reconstructing neural circuits using multiresolution correlated light and electron microscopy.
Article in Frontiers in neural circuits, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 16 citations in OpenAlex.
- Population-level geometric filtering generates motion-coherence selectivity in the retina.Science advances · 2026Article
- Metalens for passive generation of a droplet Bessel beam.Microsystems & nanoengineering · 2025Article
- Horizontal cell connectivity in the anchovy retina-a 3D electron microscopic study.BMC biology · 2025Article
- Article
- Evaluating the Quality of Serial EM Sections with Deep Learning.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2024Article
- Indirect Correlative Light and Electron Microscopy (iCLEM): A Novel Pipeline for Multiscale Quantification of Structure From Molecules to Organs.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2024Article
- Subcellular pathways through VGluT3-expressing mouse amacrine cells provide locally tuned object-motion-selective signals in the retina.Nature communications · 2024Article
- Array tomography: trails to discovery.Methods in microscopy · 2024Article
- Distributed feature representations of natural stimuli across parallel retinal pathways.Nature communications · 2024Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Correlated light and electron microscopy (CLEM) can be used to combine functional and molecular characterizations of neurons with detailed anatomical maps of their synaptic organization. Here we describe a multiresolution approach to CLEM (mrCLEM) that efficiently targets electron microscopy (EM) imaging to optically characterized cells while maintaining optimal tissue preparation for high-throughput EM reconstruction. This approach hinges on the ease with which arrays of sections collected on a solid substrate can be repeatedly imaged at different scales using scanning electron microscopy. We match this multiresolution EM imaging with multiresolution confocal mapping of the aldehyde-fixed tissue. Features visible in lower resolution EM correspond well to features visible in densely labeled optical maps of fixed tissue. Iterative feature matching, starting with gross anatomical correspondences and ending with subcellular structure, can then be used to target high-resolution EM image acquisition and annotation to cells of interest. To demonstrate this technique and range of images used to link live optical imaging to EM reconstructions, we provide a walkthrough of a mouse retinal light to EM experiment as well as some examples from mouse brain slices.
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