ReviewParasites & vectors2022
Laboratory diagnostics for human Leishmania infections: a polymerase chain reaction-focussed review of detection and identification methods.
Review in Parasites & vectors, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers, 3 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
40 citing papers in PubMed, 3 syntheses or guidelines pooled it.
- Global Landscape of Molecular and Immunological Diagnostic Tests for Human Leishmaniasis: A Systematic Review and Meta-Analysis.Pathogens (Basel, Switzerland) · 2025Pooled it
- Leishmaniasis in deployed military populations: A systematic review and meta-analysis.PLoS neglected tropical diseases · 2025Pooled it
- "High specificity of PCR in diagnosing mucocutaneous leshminiasis: a systematic review and meta analysis".BMC infectious diseases · 2024Pooled it
- An Intriguing, Solitary Nodule on the Nose.Indian dermatology online journal · 2026Article
- An integrated molecular approach for the detection and species-level identification ofMicrobiology spectrum · 2026Article
- Integrating molecular tools into leishmaniosis surveillance: evaluation of a commercial qPCR kit in sand fly vectors.Parasites & vectors · 2026Article
- Incidence and clinical patterns of cutaneous leishmaniasis at a Tertiary hospital in Hadiya Zone, Ethiopia: a hospital-based cross-sectional study.BMC infectious diseases · 2026Article
- Diagnosis of human cutaneous leishmaniasis: comparison between ITS1-PCR and ITS1-219 next generation sequencing.BMC microbiology · 2026Article
- Analytical and clinical performance of in-house and commercial real-time PCR assays for diagnosingJournal of clinical microbiology · 2026Article
- A Reliable and Accurate In-House kDNA-Based qPCR Assay for Detection of Leishmania DNA in Microscopy-Negative Cutaneous Leishmaniasis.Acta parasitologica · 2026Article
- Emerging Nanomaterials-Based Biosensors for the Clinical and Pathological Detection of E. Coli O157:H7.Current microbiology · 2026Review
- AIR-LEISH: A Dataset of Giemsa-Stained Microscopy Images for AI-based Leishmania amastigotes Detection.Scientific data · 2026Article
- LAMP-coupled CRISPR-Cas12a assays: A promising new tool for molecular diagnosis of leishmaniasis.PLoS neglected tropical diseases · 2026Article
- Enhanced diagnostic tools for visceral leishmaniasis using kinase based antigens in Brazilian human and canine samples.Scientific reports · 2026Article
- Post-surgical seroreversion in a case of equine cutaneous leishmaniosis by Leishmania infantum.Veterinary research communications · 2026Article
- TIM, a novel molecular toolbox for the detection and identification ofMicrobiology spectrum · 2026Article
- Diverse Biological Activity of Pyrimidine Derivatives: A Review.Current drug discovery technologies · 2026Review
- Optimizing a Conventional Multiplex PCR for Simultaneous Detection of Granulomatous Skin Infection Agents:Journal of tropical medicine · 2026Article
- Diagnostic methods for protozoan diseases: a review focused on leishmaniasis, Chagas disease and malaria.Frontiers in microbiology · 2026Review
- Genetic Manipulation Tools in Leishmania: From CRISPR/Cas9 to Vaccine Strategies for Disease Control.Acta parasitologica · 2025Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Leishmania infections span a range of clinical syndromes and impact humans from many geographic foci, but primarily the world's poorest regions. Transmitted by the bite of a female sand fly, Leishmania infections are increasing with human movement (due to international travel and war) as well as with shifts in vector habitat (due to climate change). Accurate diagnosis of the 20 or so species of Leishmania that infect humans can lead to the successful treatment of infections and, importantly, their prevention through modelling and intervention programs. A multitude of laboratory techniques for the detection of Leishmania have been developed over the past few decades, and although many have drawbacks, several of them show promise, particularly molecular methods like polymerase chain reaction. This review provides an overview of the methods available to diagnostic laboratories, from traditional techniques to the now-preferred molecular techniques, with an emphasis on polymerase chain reaction-based detection and typing methods.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.