Evidence map›Paper›PMID 36335164›Full record

ArticleScientific reports2022

Label-free third harmonic generation imaging and quantification of lipid droplets in live filamentous fungi.

Tanja Pajić, Nataša V Todorović, Miroslav Živić, Stanko N Nikolić, Mihailo D Rabasović, Andrew H A Clayton, Aleksandar J Krmpot

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
2.9field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Article
  3. Increase in lipid portion ofMicrobiology (Reading, England) · 2025
    Article
  4. Biocontrol Potential of NativeJournal of fungi (Basel, Switzerland) · 2025
    Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Tanja PajićFaculty of Biology, Institute of Physiology and Biochemistry, University of Belgrade, Studentski trg 16, Belgrade, 11158, Serbia.
Nataša V TodorovićInstitute for Biological Research "Siniša Stanković", University of Belgrade, National Institute of the Republic of Serbia, Bulevar Despota Stefana 142, Belgrade, 11000, Serbia.
Miroslav ŽivićFaculty of Biology, Institute of Physiology and Biochemistry, University of Belgrade, Studentski trg 16, Belgrade, 11158, Serbia.
Stanko N NikolićInstitute of Physics Belgrade, University of Belgrade, National Institute of the Republic of Serbia, Pregrevica 118, Belgrade, 11080, Serbia.
Mihailo D RabasovićInstitute of Physics Belgrade, University of Belgrade, National Institute of the Republic of Serbia, Pregrevica 118, Belgrade, 11080, Serbia.
Andrew H A ClaytonDepartment of Physics and Astronomy, Optical Sciences Centre, School of Science, Computing and Engineering Technologies, Swinburne University of Technology, Melbourne, VIC, 3122, Australia.
Aleksandar J KrmpotInstitute of Physics Belgrade, University of Belgrade, National Institute of the Republic of Serbia, Pregrevica 118, Belgrade, 11080, Serbia. krmpot@ipb.ac.rs.ORCID 0000-0003-2751-7395
University of Belgrade · RSSwinburne University of Technology · AU

Funding

Ministarstvo Prosvete, Nauke i Tehnološkog Razvoja 451-03-68/2022-14/ 200007Ministarstvo Prosvete, Nauke i Tehnološkog Razvoja 451-03-68/2022-14/ 200178Science Fund of the Republic of Serbia 6066079
6 · The paper itself

Abstract

We report the utilization of Third-Harmonic Generation microscopy for label-free live cell imaging of lipid droplets in the hypha of filamentous fungus Phycomyces blakesleeanus. THG microscopy images showed bright spherical features dispersed throughout the hypha cytoplasm in control conditions and a transient increase in the number of bright features after complete nitrogen starvation. Colocalization analysis of THG and lipid-counterstained images disclosed that the cytoplasmic particles were lipid droplets. Particle Size Analysis and Image Correlation Spectroscopy were used to quantify the number density and size of lipid droplets. The two analysis methods both revealed an increase from 16 × 10

Indexed as

Lipid DropletsSecond Harmonic Generation MicroscopyFungiMicroscopyNitrogenNitrogen

Identifiers

PMID36335164
PMCPMC9637149
OpenAlexW4308450628

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.