ArticleScientific reports2022
Phototoxic effects of nonlinear optical microscopy on cell cycle, oxidative states, and gene expression.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 20 citations in OpenAlex.
- Longitudinal live-cell chemical imaging using coherent Raman scattering microscopy.The Analyst · 2026Review
- Multimodal Raman and Fluorescence Lifetime Imaging: A New Analytical Approach for Resolving Timing-Dependent Metabolic Recovery in Endothelial Dysfunction.Analytical chemistry · 2026Article
- Simultaneous thermal and large field-of-view two-photon imaging enables optimization of signal-to-noise.Neurophotonics · 2026Article
- Evaluation of a nanosecond dissipative soliton resonance fiber laser for multiphoton microscopy.Scientific reports · 2025Article
- PhotoFiTT: a quantitative framework for assessing phototoxicity in live-cell microscopy experiments.Nature communications · 2025Article
- Label-Free Longitudinal Imaging of Single Cell Drug Response with a 3D-Printed Cell Culture Platform.Analytical chemistry · 2025Article
- Gentle Label-Free Nonlinear Optical Imaging Relaxes Linear-Absorption-Mediated Triplet.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Metabolic regulation of key developmental events during mammalian embryogenesis.Nature cell biology · 2025Review
- Multiphoton microscopy at a microwatt level via gain-managed nonlinear amplification and pulse-picking.Biomedical optics express · 2025Article
- SRS microscopy identifies inhibition of vitellogenesis as a mediator of lifespan extension by caloric restriction inbioRxiv : the preprint server for biology · 2025Article
- Ultra-Low-Dose UV-C Photo-stimulation Promotes Neural Stem Cells Differentiation via Presenilin 1 Mediated Notch and β-Catenin Activation.Molecular neurobiology · 2024Article
- Article
- Harnessing artificial intelligence to reduce phototoxicity in live imaging.Journal of cell science · 2024Article
- Chemical Reactions in Living Systems.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- Coherent Raman scattering microscopy of lipid droplets in cells and tissues.Journal of Raman spectroscopy : JRS · 2023Article
- Viewing life without labels under optical microscopes.Communications biology · 2023Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Nonlinear optical imaging modalities, such as stimulated Raman scattering (SRS) microscopy, use pulsed-laser excitation with high peak intensity that can perturb the native state of cells. In this study, we used bulk RNA sequencing, quantitative measurement of cell proliferation, and fluorescent measurement of the generation of reactive oxygen species to assess phototoxic effects of near-IR pulsed laser radiation, at different time scales, for laser excitation settings relevant to SRS imaging. We define a range of laser excitation settings for which there was no significant change in mouse Neuro2A cells after laser exposure. This study provides guidance for imaging parameters that minimize photo-induced perturbations in SRS microscopy to ensure accurate interpretation of experiments with time-lapse imaging or with paired measurements of imaging and sequencing on the same cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.