ArticlePloS one2022
Exploring the neurogenic differentiation of human dental pulp stem cells.
Article in PloS one, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 2 of them syntheses that pooled it.
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Who cites it
30 citing papers in PubMed, 2 syntheses or guidelines pooled it, 37 citations in OpenAlex.
- Hyaluronic acid as a biomaterial for dental pulp regeneration: a systematic review of preclinical studies.Frontiers in bioengineering and biotechnology · 2025Pooled it
- The growth factor multimodality on treating human dental mesenchymal stem cells: a systematic review.BMC oral health · 2024Pooled it
- A reproducible and physiologically relevant human iPSC-derived platform for in vitro modeling of the neurocardiac junction.Molecular therapy : the journal of the American Society of Gene Therapy · 2026Article
- Lipoxin A4 and Resolvin D1 Preserve Neural Inductive Capacity of Dental Pulp Stem Cells Cultured Under Inflammatory Conditions.Cell biology international · 2026Article
- Optimizing neurogenic differentiation of dental stem cells: a critical review of induction strategies and functional validation.Frontiers in dental medicine · 2026Review
- Molecular Mechanisms and Clinical Applications of Neural Regeneration Through Dental Pulp Stem Cells.Stem cells international · 2026Review
- A Concise Review of the Impact of Photobiomodulation (PBM) on Neuronal Differentiation in Adipose-Derived Mesenchymal Stem Cells.Journal of lasers in medical sciences · 2026Review
- From human dental pulp stem cells to functional cholinergic neurons: An optimized neurogenic differentiation protocol for new approach methodologies.Journal of dental sciences · 2026Article
- Multifunctional electrospun PCL/CNT/EGCG nerve conduits with a collagen hydrogel for enhanced sciatic nerve regeneration.Journal of translational medicine · 2025Article
- Viability of Dental Pulp Derived Stem Cells After Long-Term Cryopreservation.Journal of endodontics · 2025Article
- Engineered Human Dental Pulp Stem Cells with Promising Potential for Regenerative Medicine.Biotech (Basel (Switzerland)) · 2025Review
- Advances in the Research of Mesenchymal Stromal Cells in the Treatment of Maxillofacial Neurological Disorders and the Promotion of Facial Nerve Regeneration.Molecular neurobiology · 2025Review
- Neuronal Differentiation and Exosome Profiling of Dental Pulp Stem Cells: Unveiling Their Potential for Nerve Repair.International journal of molecular sciences · 2025Article
- Isolation methods of exosomes derived from dental stem cells.International journal of oral science · 2025Review
- The role of neurogenic inflammation in pulp repair and the techniques used for its assessment (narrative review).Frontiers in dental medicine · 2025Review
- Wnt/CaJournal of dental sciences · 2024Article
- Unveiling the Neurodegenerative Alterations through Oral Stem Cells.Journal of dental research · 2024Article
- ARMCX3 regulates ROS signaling, affects neural differentiation and inflammatory microenvironment in dental pulp stem cells.Heliyon · 2024Article
- Recent Advances in Hydrogel Technology in Delivering Mesenchymal Stem Cell for Osteoarthritis Therapy.Biomolecules · 2024Review
- Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human dental pulp stem cells (hDPSCs) have increasingly gained interest as a potential therapy for nerve regeneration in medicine and dentistry, however their neurogenic potential remains a matter of debate. This study aimed to characterize hDPSC neuronal differentiation in comparison with the human SH-SY5Y neuronal stem cell differentiation model. Both hDPSCs and SH-SY5Y could be differentiated to generate typical neuronal-like cells following sequential treatment with all-trans retinoic acid (ATRA) and brain-derived neurotrophic factor (BDNF), as evidenced by significant expression of neuronal proteins βIII-tubulin (TUBB3) and neurofilament medium (NF-M). Both cell types also expressed multiple neural gene markers including growth-associated protein 43 (GAP43), enolase 2/neuron-specific enolase (ENO2/NSE), synapsin I (SYN1), nestin (NES), and peripherin (PRPH), and exhibited measurable voltage-activated Na+ and K+ currents. In hDPSCs, upregulation of acetylcholinesterase (ACHE), choline O-acetyltransferase (CHAT), sodium channel alpha subunit 9 (SCN9A), POU class 4 homeobox 1 (POU4F1/BRN3A) along with a downregulation of motor neuron and pancreas homeobox 1 (MNX1) indicated that differentiation was more guided toward a cholinergic sensory neuronal lineage. Furthermore, the Extracellular signal-regulated kinase 1/2 (ERK1/2) inhibitor U0126 significantly impaired hDPSC neuronal differentiation and was associated with reduction of the ERK1/2 phosphorylation. In conclusion, this study demonstrates that extracellular signal-regulated kinase/Mitogen-activated protein kinase (ERK/MAPK) is necessary for sensory cholinergic neuronal differentiation of hDPSCs. hDPSC-derived cholinergic sensory neuronal-like cells represent a novel model and potential source for neuronal regeneration therapies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.