Evidence map›Paper›PMID 36331762›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Isolate and Culture Mouse Primary Neurons for West Nile Virus Infection.

Farzana Nazneen, Fengwei Bai

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Farzana NazneenDepartment of Cell and Molecular Biology, Center for Molecular and Cellular Biosciences, The University of Southern Mississippi, Hattiesburg, MS, USA.
Fengwei BaiDepartment of Cell and Molecular Biology, Center for Molecular and Cellular Biosciences, The University of Southern Mississippi, Hattiesburg, MS, USA. fengwei.bai@usm.edu.

Funding

Training and Mentoring Core P20GM103476 · NIGMS · UNIVERSITY OF SOUTHERN MISSISSIPPI · PI MICHAEL R GARRETT · 2012 to 2026
$60.2M
Interleukin-17A promotes CD8+ T cell responses in West Nile virus infectionR15AI135893 · NIAID · UNIVERSITY OF SOUTHERN MISSISSIPPI · PI BAI, FENGWEI · 2019 to 2019
$444k
Validation of the Regulatory Potential of Tailed MirtronsR15GM120716 · NIGMS · UNIVERSITY OF SOUTHERN MISSISSIPPI · PI FLYNT, ALEX · 2016 to 2016
$437k
NIAID NIH HHS R15 AI135893NIGMS NIH HHS P20 GM103476NIGMS NIH HHS R15 GM120716
6 · The paper itself

Abstract

Primary neurons are very valuable cells to study the pathogenesis of neurotropic viruses, such as West Nile virus. The mouse primary neurons can be used to assess viral infection profiles and cellular immune responses to a viral infection. However, successful isolation and culture of mouse neurons can be challenging. Here, we report a step-by-step method to prepare a primary neuron culture from adult mice.

Indexed as

West Nile FeverWest Nile virusAnimalsImmunity, CellularMiceNeuronsCulture protocolMouse primary neuronNeurotrophic virusWest Nile virus

Identifiers

PMID36331762
PMCPMC10719967

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.