ArticlePlant physiology2023
MdbHLH4 negatively regulates apple cold tolerance by inhibiting MdCBF1/3 expression and promoting MdCAX3L-2 expression.
Article in Plant physiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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33 citing papers in PubMed, 59 citations in OpenAlex.
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- The ApCtf1β2-ApCUT3 Module Mediates Host Cuticle Penetration and Modulates Immune Responses Thereby Influencing Blight Resistance in Bambusa pervariabilis × Dendrocalamopsis grandis.Plant, cell & environment · 2026Article
- Engineering climate-resilient horticultural crops: advances in transcriptional regulation, genome editing, and synthetic networks.Horticulture research · 2026Article
- Regulatory insights underlying apple to cold stress.Horticulture research · 2026Article
- A Hierarchical VvbHLH30-VvERF70-VvACS2 Module Orchestrates Ethylene Biosynthesis and Cold Adaptation in Grapevine.Plant biotechnology journal · 2026Article
- AaSIZ1-Mediated SUMOylation of AaMYB31 Positively Regulates Freezing Tolerance in Actinidia arguta.Plant biotechnology journal · 2026Article
- Cold-responsive interaction between MdRAD23D1 and MdMYB15 confers cold stress tolerance via the CBF pathway in apple (Malus domestica).PLoS genetics · 2026Article
- Genome-Wide Analysis ofInternational journal of molecular sciences · 2026Article
- Identification of the cation/H+ exchanger genes in peanut and functional analysis ofFrontiers in plant science · 2026Article
- Molecular mechanisms of plant freezing tolerance: from cold signal perception to adaptive responses.Frontiers in plant science · 2026Review
- CAX1 beyond calcium transport: evidence for a transport-independent regulatory module in plants.Frontiers in plant science · 2026Review
- A SIP1 gene of Trihelix family, SlGT-33, promotes chilling tolerance and fruits set under prolonged cold stress in tomatoes.BMC plant biology · 2025Article
- Evolution and diversification of CaBMC plant biology · 2025Article
- The MdHB7L-MdICE1L-MdHOS1 Module Fine-Tunes Apple Cold Response via CBF-Dependent and CBF-Independent Pathways.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Genome-Wide Identification and Expression Divergence ofLife (Basel, Switzerland) · 2025Article
- MdVQ37 Negatively Regulates Apple Resistance to Valsa Canker via SA-Dependent and SA-Independent Pathways.Molecular plant pathology · 2025Article
- Plant Coping with Cold Stress: Molecular and Physiological Adaptive Mechanisms with Future Perspectives.Cells · 2025Review
- Candidate genes associated with low temperature tolerance in cucumber adult plants identified by combining GWAS & QTL mapping.Stress biology · 2024Article
- Ectopic expression of HvbHLH132 from hulless barley reduces cold tolerance in transgenic Arabidopsis thaliana.Plant cell reports · 2024Article
- Identification of MYC genes in four Cucurbitaceae species and their roles in the response to temperature stress.BMC genomics · 2024Article
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Authors and funding
8 authors at 1 institution in 1 country.
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Abstract
Low temperature affects the yield and quality of crops. Inducer of CBF expression 1 (ICE1) plays a positive role in plant cold tolerance by promoting the expression of CRT binding factor (CBF) and cold-responsive (COR) genes. Several ICE1-interacting transcription factors (TFs) that regulate plant cold tolerance have been identified. However, how these TFs affect the function of ICE1 and CBF expression under cold conditions remains unclear. Here, we identified the MYC-type TF MdbHLH4, a negative regulator of cold tolerance in Arabidopsis (Arabidopsis thaliana) and apple (Malus domestica) plants. Under cold conditions, MdbHLH4 inhibits the expression of MdCBF1 and MdCBF3 by directly binding to their promoters. It also interacts with MdICE1L, a homolog of AtICE1 in apple, and inhibits the binding of MdICE1L to the promoters of MdCBF1/3 and thus their expression. We showed that MdCAX3L-2, a Ca2+/H+ exchanger (CAX) family gene that negatively regulates plant cold tolerance, is also a direct target of MdbHLH4. MdbHLH4 reduced apple cold tolerance by promoting MdCAX3L-2 expression. Moreover, overexpression of either MdCAX3L-2 or MdbHLH4 promoted the cold-induced ubiquitination and degradation of MdICE1L. Overall, our results reveal that MdbHLH4 negatively regulates plant cold tolerance by inhibiting MdCBF1/3 expression and MdICE1L promoter-binding activity, as well as by promoting MdCAX3L-2 expression and cold-induced MdICE1L degradation. These findings provide insights into the mechanisms by which ICE1-interacting TFs regulate CBF expression and ICE1 function and thus plant cold tolerance.
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