Evidence map›Paper›PMID 36329579›Full record

ArticleNan fang yi ke da xue xue bao = Journal of Southern Medical University2022

[Numb activates the mTORC1 signaling pathway in proximal tubular epithelial cells by upregulating V1G1 expression].

Z Liu, D You, Y Li, Y He, A Li, P Li, C Li

Open access · greenAbstract readEnglish Abstract
PubMed Full text at PMC Publisher
In one paragraph

Article in Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Z LiuSchool of Nursing, Xiangnan University, Chenzhou 423000, China.
D YouSchool of Clinical Medicine, Xiangnan University, Chenzhou 423000, China.
Y LiSchool of Nursing, Xiangnan University, Chenzhou 423000, China.
Y HeSchool of Nursing, Xiangnan University, Chenzhou 423000, China.
A LiSchool of Nursing, Xiangnan University, Chenzhou 423000, China.
P LiSchool of Nursing, Xiangnan University, Chenzhou 423000, China.
C LiSchool of Nursing, Xiangnan University, Chenzhou 423000, China.
Xiangnan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo investigate the role of Numb in regulating mammalian target of rapamycin (mTOR) complex 1 (mTORC1) signaling pathway.

methodsMale BALB/C mouse models of acute kidney injury (AKI) were subjected to intravenous injections of Numb-siRNA or NC-siRNA with or without intraperitoneal cisplatin injections. After the treatments, the expressions and distribution of Numb and megalin in the renal tissues of the mice were detected with immunohistochemistry, and the renal expressions of Numb, S6, p-S6, S6K1, p-S6K1, 4EBP1 and p-4EBP1 were examined with Western blotting. The proximal renal tubular epithelial cells were isolated from the mice transfected with Numb-siRNA for in vitro culture. In NRK-52E cells, the effects of amino acid stimulation, Numb knockdown, and V1G1 overexpression, alone or in combination, on expressions of Numb, S6 and p-S6 were detected with Western blotting; the expressions of AMPK and p-AMPK were also detected in transfected NRK-52E cells, mouse kidneys and cultured mouse renal tubular epithelial cells.

resultsIn BALB/C mice, injection of Numb-siRNA caused significant reductions of Numb and p-S6 expressions without affecting megalin expression in the renal proximal tubules (

conclusionNumb promotes the activation of mTORC1 signaling in proximal tubular epithelial cells by upregulating V1G1 expression.

Indexed as

CisplatinLow Density Lipoprotein Receptor-Related Protein-2Amino AcidsAMP-Activated Protein KinasesAnimalsEpithelial CellsMaleMammalsMechanistic Target of Rapamycin Complex 1Membrane ProteinsMiceMice, Inbred BALB CNerve Tissue ProteinsRNA, Small InterferingSignal TransductionVacuolar Proton-Translocating ATPasesAmino AcidsAMP-Activated Protein KinasesATP6V1G1 protein, mouseCisplatinLow Density Lipoprotein Receptor-Related Protein-2Mechanistic Target of Rapamycin Complex 1Membrane ProteinsNerve Tissue ProteinsNumb protein, mouseRNA, Small InterferingVacuolar Proton-Translocating ATPasesAMP activated protein kinasmTOR complex 1NRK-52E cellsNumbV1G1

Identifiers

PMID36329579
PMCPMC9637490
OpenAlexW4308553059

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.