Evidence map›Paper›PMID 36329326›Full record

ArticleCurrent microbiology2022

The Pseudogene BMEA_B0173 Deficiency in Brucella melitensis Contributes to M-epitope Formation and Potentiates Virulence in a Mice Infection Model.

Ge Zhang, Hao Dong, Yu Feng, Hui Jiang, Tonglei Wu, Jiali Sun, Xin Wang, Minghe Liu, Xiaowei Peng, Yinghui Zhang and 4 more

Abstract read
PubMed Publisher
In one paragraph

Article in Current microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 5 institutions in 1 country.

Ge Zhang *College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, 212018, China.
Hao Dong *Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control, Beijing, China.
Yu FengNational Reference Laboratory for Animal Brucellosis, China Institute of Veterinary Drug Control, Beijing, China.
Hui JiangInsititute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Tonglei WuCollege of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao, China.
Jiali SunNational Reference Laboratory for Animal Brucellosis, China Institute of Veterinary Drug Control, Beijing, China.
Xin WangNational Reference Laboratory for Animal Brucellosis, China Institute of Veterinary Drug Control, Beijing, China.
Minghe LiuNational Reference Laboratory for Animal Brucellosis, China Institute of Veterinary Drug Control, Beijing, China.
Xiaowei PengNational Reference Laboratory for Animal Brucellosis, China Institute of Veterinary Drug Control, Beijing, China.
Yinghui ZhangNational Reference Laboratory for Animal Brucellosis, China Institute of Veterinary Drug Control, Beijing, China.
Xiaoqian ZhangNational Reference Laboratory for Animal Brucellosis, China Institute of Veterinary Drug Control, Beijing, China.
Liangquan ZhuNational Reference Laboratory for Animal Brucellosis, China Institute of Veterinary Drug Control, Beijing, China.
Jiabo DingInsititute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. dingjiabo@126.com.
Xingjia ShenCollege of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, 212018, China. shenxjsri@163.com.
China Institute of Veterinary Drug Control · CNChinese Academy of Agricultural Sciences · CNHebei Normal University of Science and Technology · CNJiangsu University of Science and Technology · CNNational Institutes for Food and Drug Control · CN

Funding

National Key Research and Development Program of China 2016YFD0500902Natural Science Foundation of China 31902310
6 · The paper itself

Abstract

It is widely acknowledged that pseudogenes play important roles in bacterial diversification and evolution and participate in gene regulation and RNA interference (RNAi). However, the function of most pseudogenes in Brucella spp remains poorly understood, warranting further studies.To comprehensively analyze the function of the pseudogenes BMEA_B0173 in Brucella melitensis strain 63/9, a BMEA_B0173 in-frame deleted mutant strain was constructed. Then, the phenotypes of the mutant strain, such as growth characteristics and bacterial virulence, were assessed in mice infection models. Finally, iTRAQ analysis was performed to investigate the gene expression profile affected by the pseudogenes BMEA_B0173. In this study, we found that BMEA_B0173 deletion exhibited increased agglutination with M monospecific sera. In a mouse model of chronic infection, the BMEA_B0173 deletion strain displayed increased colonization in the spleen compared to the wild-type pathogen. The iTRAQ assay revealed that 252 proteins were differentially expressed between the BMEA_B0173 deletion and the wild-type strains. In addition, deletion of BMEA_B0173 significantly increased the expression of proteins involved in the denitrification pathway, iron metabolism, and several transcriptional regulators, which might cause increased virulence of the mutant strain. In conclusion, this study preliminary uncovered the function of the pseudogene BMEA_B0173 in Brucella melitensis 63/9 and provided novel insights for studying the pathogenesis of Brucella strains.

Indexed as

Brucella melitensisBrucellosisAnimalsBacterial ProteinsDisease Models, AnimalEpitopesMicePseudogenesVirulenceBacterial ProteinsEpitopes

Identifiers

PMID36329326
OpenAlexW4308368205

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.