Evidence map›Paper›PMID 36328771›Full record

ArticleSmall GTPases2022

A New Crosslinking Assay to Study Guanine Nucleotide Binding in the Gtr Heterodimer of

Dylan D Doxsey, Kristen Veinotte, Kuang Shen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Small GTPases, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Dylan D DoxseyProgram in Molecular Medicine, University of Massachusetts Chan Medical School, 373 Plantation St, Worcester, MA, 01605, USA.
Kristen VeinotteProgram in Molecular Medicine, University of Massachusetts Chan Medical School, 373 Plantation St, Worcester, MA, 01605, USA.
Kuang ShenProgram in Molecular Medicine, University of Massachusetts Chan Medical School, 373 Plantation St, Worcester, MA, 01605, USA.ORCID 0000-0002-0873-758X

Funding

Spatial and temporal regulation of nutrient sensingR35GM146824 · NIGMS · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI Kuang Shen · 2022 to 2026
$2.2M
American Heart Association-American Stroke Association 928304NIGMS NIH HHS R35 GM146824
6 · The paper itself

Abstract

The mechanistic target of rapamycin (mTOR) complex is responsible for coordinating nutrient availability with eukaryotic cell growth. Amino acid signals are transmitted towards mTOR via the Rag/Gtr heterodimers. Due to the obligatory heterodimeric architecture of the Rag/Gtr GTPases, investigating their biochemical properties has been challenging. Here, we describe an updated assay that allows us to probe the guanine nucleotide-binding affinity and kinetics to the Gtr heterodimers in

Indexed as

Monomeric GTP-Binding ProteinsSaccharomyces cerevisiaeGTP PhosphohydrolasesGuanineGuanine NucleotidesHumansMechanistic Target of Rapamycin Complex 1NucleotidesTOR Serine-Threonine KinasesGTP PhosphohydrolasesGuanineGuanine NucleotidesMechanistic Target of Rapamycin Complex 1Monomeric GTP-Binding ProteinsNucleotidesTOR Serine-Threonine Kinasescrosslinkingenzyme kineticsGTPaseGtr GTPaseguanine nucleotidemTORnutrient sensingRag GTPase

Identifiers

PMID36328771
PMCPMC9639563

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.