ArticleJournal of clinical microbiology2022
A Practical Workflow for the Identification of Aspergillus, Fusarium, Mucorales by MALDI-TOF MS: Database, Medium, and Incubation Optimization.
Article in Journal of clinical microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Reduced serum iron levels predict poor prognosis in pulmonary mucormycosis patients: a prospective, case‒control study.Scientific reports · 2025Trial
- Establishment of a matrix-assisted laser desorption/ionization time-of-flight mass spectrometry database and evaluation of different methods for cluster analysis of marine bacteria.Journal of medical microbiology · 2026Article
- Evaluation of MALDI-TOF MS for identification of clinical filamentous fungi in a routine mycology laboratory.BMC microbiology · 2026Article
- Optimizing filamentous fungi identification by MALDI-TOF MS: A comparative analysis of key factors.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- Comparative performance of Filamentous Fungi 4.0 and Mass Spectrometry Identification 2.0 databases for Aspergillus spp.: identification using a simplified protein extraction method.Revista do Instituto de Medicina Tropical de Sao Paulo · 2026Article
- Species distribution, antifungal susceptibility, and clinical profiles of patients with osteoarticular fungal Infections: A retrospective study.New microbes and new infections · 2025Article
- Trend Analysis of Fungal Infections Based on Culture among Inpatients in Henan Province from 2018 to 2023.Polish journal of microbiology · 2025Article
- Spectral Precision: Recent Advances in Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for Pathogen Detection and Resistance Profiling.Microorganisms · 2025Review
- Comparison of MALDI-TOF MS instruments and databases for the identification of uncommon yeasts,Journal of clinical microbiology · 2025Article
- Comparison of the Filamentous Fungi Library v4.0 MALDI Biotyper Platform vs MSI-2 performance for identifying filamentous fungi from liquid cultures.Journal of clinical microbiology · 2025Article
- Mucormycosis.Infectious disease clinics of North America · 2025Review
- Comparison of the BluePoint MoldID oligonucleotide array and Bruker Biotyper MALDI-TOF MS for the identification of filamentous fungi.Journal of clinical microbiology · 2025Article
- Exploring fungal diversity in Vietnam: A citizen science initiative.One health (Amsterdam, Netherlands) · 2024Article
- What do We Know about Cryptic Aspergillosis?Microorganisms · 2024Review
- Laboratory and clinical management capacity for invasive fungal infections: the Italian landscape.Infection · 2024Article
- Invasive fusariosis.Clinical microbiology reviews · 2023Review
- A comparative study for optimization of MALDI-TOF MS identification of filamentous fungi.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2023Article
- Comparative genomics predict specific genes in potential mucorales identification.Archives of microbiology · 2023Article
- Spectrometric Characterization of Clinical and Environmental Isolates ofJournal of fungi (Basel, Switzerland) · 2023Article
- Evaluation of a Novel Benchtop Tool for Acceleration of Sample Preparation for MALDI-TOF Mass Spectrometry.Journal of clinical microbiology · 2023Article
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9 authors.
Funding
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Abstract
There is an increasing body of literature on the utility of MALDI-TOF MS in the identification of filamentous fungi. However, the process still lacks standardization. In this study, we attempted to establish a practical workflow for the identification of three clinically important molds: Aspergillus, Fusarium, and Mucorales using MALDI-TOF MS. We evaluated the performance of Bruker Filamentous Fungi database v3.0 for the identification of these fungi, highlighting when there would be a benefit of using an additional database, the MSI-2 for further identification. We also examined two other variables, namely, medium effect and incubation time on the accuracy of fungal identification. The Bruker database achieved correct species level identification in 85.7% of Aspergillus and 90% of Mucorales, and correct species-complex level in 94.4% of Fusarium. Analysis of spectra using the MSI-2 database would also offer additional value for species identification of Aspergillus species, especially when suspecting species with known identification limits within the Bruker database. This issue would only be of importance in selected cases where species-level identification would impact therapeutic options. Id-Fungi plates (IDFP) had almost equivalent performance to Sabouraud dextrose agar (SDA) for species-level identification of isolates and enabled an easier harvest of the isolates with occasional faster identification. Our study showed accurate identification at 24 h for Fusarium and Mucorales species, but not for Aspergillus species, which generally required 48 h.
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