ArticleFrontiers in bioengineering and biotechnology2022
Enhanced small green fluorescent proteins as a multisensing platform for biosensor development.
Article in Frontiers in bioengineering and biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Characterization of bovine-derived H5N1 viruses expressing fluorescent and luminescent reporter proteins.The Journal of general virology · 2026Article
- The proteinaceous biofilm ofBiofilm · 2026Article
- Advances in fluorescence-based point-of-care diagnostics: probes, nanostructures and integrated systems.Journal of materials chemistry. C · 2026Review
- Highly efficient expression of DNA-peptide conjugates in growth-arrested cells.Nature communications · 2026Article
- BRIGHT Enables High-SNR Live-Cell Imaging of Non-Repetitive Sequences via Bivalent Fluorescent Nanobody-Mediated Cascade-Dependent Illumination.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Bathochromic Shift via C=O to C=S Substitution: A Far-Red Fluorogen for Multiplexed FLIM with FAST Fluorogen-Activating Protein.International journal of molecular sciences · 2025Article
- Ligase-mediated programmable genomic integration (L-PGI).Nature communications · 2025Article
- Insertion of Fluorescent Proteins Near the Plug Domain of MotB Generates Functional Stator Complexes.MicrobiologyOpen · 2025Article
- Genetically encoded green fluorescent sensor for probing sulfate transport activity of solute carrier family 26 member a2 (Slc26a2) protein.Communications biology · 2024Article
- Fluorescent protein tags affect the condensation properties of a phase-separating viral protein.Molecular biology of the cell · 2024Article
- Rational Design of a Circularly Permuted Flavin-Based Fluorescent Protein.Chembiochem : a European journal of chemical biology · 2024Article
- Fluorescent Protein-Based Sensors for Detecting Essential Metal Ions across the Tree of Life.ACS sensors · 2024Review
- Reengineering of a flavin-binding fluorescent protein using ProteinMPNN.Protein science : a publication of the Protein Society · 2024Article
- NeMeHg, genetically encoded indicator for mercury ions based on mNeonGreen green fluorescent protein and merP protein fromFrontiers in bioengineering and biotechnology · 2024Article
- Backbone Cyclization of Flavin Mononucleotide-Based Fluorescent Protein Increases Fluorescence and Stability.Journal of microbiology and biotechnology · 2023Article
- Anaerobic fluorescent reporters for live imaging ofFrontiers in microbiology · 2023Article
- Electrochemical biosensors in healthcare services: bibliometric analysis and recent developments.PeerJ · 2023Review
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Engineered light, oxygen, and voltage (LOV)-based proteins are able to fluoresce without oxygen requirement due to the autocatalytic incorporation of exogenous flavin as a chromophore thus allowing for live cell imaging under hypoxic and anaerobic conditions. They were also discovered to have high sensitivity to transition metal ions and physiological flavin derivatives. These properties make flavin-binding fluorescent proteins (FPs) a perspective platform for biosensor development. However, brightness of currently available flavin-binding FPs is limited compared to GFP-like FPs creating a need for their further enhancement and optimization. In this study, we applied a directed molecular evolution approach to develop a pair of flavin-binding FPs, named miniGFP1 and miniGFP2. The miniGFP proteins are characterized by cyan-green fluorescence with excitation/emission maxima at 450/499 nm and a molecular size of ∼13 kDa. We carried out systematic benchmarking of miniGFPs in
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