Evidence map›Paper›PMID 36324745›Full record

ArticleFrontiers in cardiovascular medicine2022

Extracellular vesicles produced by human-induced pluripotent stem cell-derived endothelial cells can prevent arterial stenosis in mice via autophagy regulation.

Yecheng He, Quanfu Li, Feng Feng, Rupan Gao, Huadong Li, Yuxin Chu, Shaobo Li, Yin Wang, Ruoying Mao, Zhongzhong Ji and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in cardiovascular medicine, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Yecheng HeDepartment of Clinical Medicine, Suzhou Vocational Health College, Suzhou, Jiangsu, China.
Quanfu LiDepartment of Anesthesiology, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China.
Feng FengInstitute of Physical Education, Inner Mongolia Normal University, Hohhot, Inner Mongolia, China.
Rupan GaoDepartment of Hematology, Zhongshan Hospital, Shanghai Medical College of Fudan University, Shanghai, China.
Huadong LiDepartment of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
Yuxin ChuDepartment of Medicine, University of Alabama at Birmingham, Birmingham, AL, United States.
Shaobo LiSchool of Life Sciences, Westlake University, Hangzhou, Zhejiang, China.
Yin WangSchool of Life Sciences, Westlake University, Hangzhou, Zhejiang, China.
Ruoying MaoSchool of Life Sciences, Westlake University, Hangzhou, Zhejiang, China.
Zhongzhong JiSchool of Life Sciences, Westlake University, Hangzhou, Zhejiang, China.
Yutao HuaDepartment of Medicine, University of Alabama at Birmingham, Birmingham, AL, United States.
Jun ShenDepartment of Pharmacy, Suzhou Vocational Health College, Suzhou, Jiangsu, China.
Ziao WangSchool of Life Sciences, Xiamen University, Xiamen, Fujian, China.
Meng ZhaoSchool of Life Sciences, Westlake University, Hangzhou, Zhejiang, China.
Qing YaoHubei Key Laboratory of Diabetes and Angiopathy, Medicine Research Institute, Xianning Medical College, Hubei University of Science and Technology, Xianning, Hubei, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Intravascular transplantation of human-induced pluripotent stem cells (hiPSCs) demonstrated a significant therapeutic effect in the treatment of restenosis by the paracrine function of extracellular vesicles (EVs). However, the risk of tumorigenicity and poor cell survival limits its clinical applications. In this study, we for the first time applied a highly efficient and robust three-dimensional (3D) protocol for hiPSC differentiation into endothelial cells (ECs) with subsequent isolation of EVs from the derived hiPSC-EC (ECs differentiated from hiPSCs), and validated their therapeutic effect in intimal hyperplasia (IH) models. We found that intravenously (iv) injected EVs could accumulate on the carotid artery endothelium and significantly alleviate the intimal thickening induced by the carotid artery ligation. To elucidate the mechanism of this endothelial protection, we performed miRNA expression profiling and found out that among the most conserved endothelial miRNAs, miR-126 was the most abundant in hiPSC-EC-produced EVs (hiPSC-EC-EV). MiR-126 depletion from hiPSC-EC-EV can hinder its protective effect on human umbilical vein endothelial cells (HUVECs) in an inflammatory process. A variety of functional

Indexed as

arterial restenosisautophagyextracellular vesicleshiPSC-ECmiR-126

Identifiers

PMID36324745
PMCPMC9618599

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.