Evidence map›Paper›PMID 36324326›Full record

ArticleOpen forum infectious diseases2022

Pooling Rectal, Pharyngeal, and Urine Samples to Detect

Thierry Prazuck, Philippe Lanotte, Gwénaël Le Moal, Laurent Hocqueloux, Simon Sunder, Mélanie Catroux, Magali Garcia, Pascale Perfezou, Guillaume Gras, Chloé Plouzeau and 2 more

Registry-linked trialAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Open forum infectious diseases, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT05872438 (Evaluation of Multisite Sampling to Detect C. Trachomatis or N. Gonorrheae Compared With Vaginal Sampling in Women at Risk for Sexually Transmitted Infections), which is not on this map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT05872438 nacompletednot on this mapstarted 2023, after this paper: background citation

Evaluation of Multisite Sampling to Detect C. Trachomatis or N. Gonorrheae Compared With Vaginal Sampling in Women at Risk for Sexually Transmitted Infections (STI)

TypeinterventionalSponsorCentre Hospitalier Régional d'OrléansRan2023 to 2024Enrolled1,500ConditionsSexually Transmitted InfectionArmsVaginal, Oral and anal sampling
3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Thierry PrazuckService des maladies infectieuses et tropicales, CHR Orléans, Orleans, France.
Philippe LanotteService de bacteriologie-virologie, Centre Hospitalier Universitaire Tours, Tours, France.
Gwénaël Le MoalService des maladies infectieuses, Centre Hospitalier Universitaire Poitiers, Poitiers, France.
Laurent HocquelouxService des maladies infectieuses et tropicales, CHR Orléans, Orleans, France.ORCID https://orcid.org/0000-0002-2264-4822
Simon SunderService des maladies infectieuses, CH Niort, Niort, France.
Mélanie CatrouxService des maladies infectieuses, Centre Hospitalier Universitaire Poitiers, Poitiers, France.
Magali GarciaLaboratoire inflammation tissus épitheliaux et cytokines EA 4331, Université de Poitiers, Poitiers, France.
Pascale PerfezouService des maladies infectieuses, CH Quimper, Quimper, France.
Guillaume GrasService des maladies infectieuses, Centre Hospitalier Universitaire Tours, Tours, France.
Chloé PlouzeauLaboratoire de bactériologie, Centre Hospitalier Universitaire Poitiers, Poitiers, France.
Nicolas LévêqueLaboratoire inflammation tissus épitheliaux et cytokines EA 4331, Université de Poitiers, Poitiers, France.
Agnès Beby-DefauxLaboratoire de virologie et mycobactériologie, Centre Hospitalier Universitaire Poitiers, Poitiers, France.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Screening for Methods: In this multicenter study, MSM with a positive result for CT, NG, or MG were recalled to the clinic for treatment and were asked to participate in this study. Separate samples were sent to a central virological department that proceeded to form the pooled samples. Testing was performed using the multiplex real-time polymerase chain reaction Allplex STI Essential Assay (Seegene, Seoul, Korea), which can simultaneously detect 7 pathogens. Results: A total of 130 MSM with at least 1 positive test for CT, NG, or MG were included. A total of 25.4% had a coinfection. The sensitivities of pooled-sample testing were 94.8% for CT, 97.0% for NG, and 92.3% for MG. Pooling failed to detect 8 infections, but pooled-sample analysis missed detecting only samples with a low bacterial load (cycle threshold >35). Conclusions: Pooling samples from MSM to detect CT, NG, and MG is as sensitive as individual-site testing for these 3 pathogens using the Allplex assay. Missed infections with a very low bacterial load could have a low impact on further transmission.

Indexed as

Chlamydia trachomatisMycoplasma genitaliumNeisseria gonorrhoeaepooled samplessexually transmitted infections

Identifiers

PMID36324326
PMCPMC9620425

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Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.