Evidence map›Paper›PMID 36324122›Full record

ArticleBMC musculoskeletal disorders2022

Nutritional deficiency induces nucleus pulposus cell apoptosis via the ATF4-PKM2-AKT signal axis.

Ningfeng Zhou, Bin Shen, Chong Bai, Liang Ma, Shanjin Wang, Desheng Wu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC musculoskeletal disorders, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.9field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 6 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Ningfeng Zhou *Department of Spinal Surgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.
Bin Shen *Department of Spinal Surgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.
Chong Bai *Department of Spinal Surgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.
Liang MaDepartment of Spinal Surgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.
Shanjin WangDepartment of Spinal Surgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China. kingspine@163.com.
Desheng WuDepartment of Spinal Surgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China. eastspinesci@163.com.
Shanghai East Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe intervertebral disc is the largest avascular tissue in the human body. The nucleus pulposus (NP) consumes glucose and oxygen to generate energy to maintain cellular metabolism via nutrients that diffuse from the cartilage endplate. The microenvironment in the intervertebral disc becomes nutritionally deficient during degeneration, and nutritional deficiency has been shown to inhibit the viability and proliferation of NP cells.

methodsTo investigate the molecular mechanism by which nutritional deficiency reduces viability and decreases proliferation, we created an in vitro model by using decreasing serum concentration percentages.

resultsIn this study, we found that nutritional deficiency reduced NP cell viability and increased cell apoptosis and that the upregulation of ATF4 expression and the downregulation of PKM2 expression were involved in this process. Moreover, we found that PKM2 inhibition can reduce the cell apoptosis induced by ATF4 silence under nutritional deficiency.

conclusionOur findings revealed that PKM2 inhibition reduces the cell apoptosis induced by ATF4 silence under nutritional deficiency by inhibiting AKT phosphate. Revealing the function and mechanism of NP cell development under nutritional deficiency will provide new insights into the etiology, diagnosis, and treatment of intervertebral disc and related diseases.

Indexed as

Intervertebral Disc DegenerationMalnutritionNucleus PulposusActivating Transcription Factor 4ApoptosisHumansProto-Oncogene Proteins c-aktThyroid Hormone-Binding ProteinsActivating Transcription Factor 4ATF4 protein, humanProto-Oncogene Proteins c-aktThyroid Hormone-Binding ProteinsATF4Intervertebral disc degenerationNucleus pulposusNutritional deficiencyPKM2

Identifiers

PMID36324122
PMCPMC9628105
OpenAlexW4308142594

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.