Evidence map›Paper›PMID 36322091›Full record

ArticleStem cells translational medicine2022

Schwann Cells in the Aganglionic Colon of Hirschsprung Disease Can Generate Neurons for Regenerative Therapy.

Weikang Pan, Ahmed A Rahman, Rhian Stavely, Sukhada Bhave, Richard Guyer, Meredith Omer, Nicole Picard, Allan M Goldstein, Ryo Hotta

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells translational medicine, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
6.3field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 30 citations in OpenAlex.

  1. Enteric nervous system as a therapeutic target in gastrointestinal disorders.World journal of gastrointestinal pharmacology and therapeutics · 2025
    Article
  2. Review
  3. Article
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  5. Article
  6. Surgical pathology of Hirschsprung disease (HSCR).World journal of pediatric surgery · 2025
    Review
  7. Review
  8. Article
  9. Review
  10. Article
  11. Article
  12. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 2 countries.

Weikang PanDepartment of Pediatric Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
Ahmed A RahmanDepartment of Pediatric Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
Rhian StavelyDepartment of Pediatric Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
Sukhada BhaveDepartment of Pediatric Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
Richard GuyerDepartment of Pediatric Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.ORCID 0000-0001-5075-1767
Meredith OmerDepartment of Pediatric Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
Nicole PicardDepartment of Pediatric Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
Allan M GoldsteinDepartment of Pediatric Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
Ryo HottaDepartment of Pediatric Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.ORCID 0000-0001-9890-8241
Harvard University · US

Funding

Characterizing neurogenic progenitors in the adult intestineR01DK119210 · NIDDK · MASSACHUSETTS GENERAL HOSPITAL · PI ALLAN M GOLDSTEIN · 2020 to 2026
$2.5M
NIDDK NIH HHS R01 DK119210
6 · The paper itself

Abstract

Cell therapy offers the potential to replace the missing enteric nervous system (ENS) in patients with Hirschsprung disease (HSCR) and to restore gut function. The Schwann cell (SC) lineage has been shown to generate enteric neurons pre- and post-natally. Here, we aimed to isolate SCs from the aganglionic segment of HSCR and to determine their potential to restore motility in the aganglionic colon. Proteolipid protein 1 (PLP1) expressing SCs were isolated from the extrinsic nerve fibers present in the aganglionic segment of postnatal mice and patients with HSCR. Following 7-10 days of in vitro expansion, HSCR-derived SCs were transplanted into the aganglionic mouse colon ex vivo and in vivo. Successful engraftment and neuronal differentiation were confirmed immunohistochemically and calcium activity of transplanted cells was demonstrated by live cell imaging. Organ bath studies revealed the restoration of motor function in the recipient aganglionic smooth muscle. These results show that SCs isolated from the aganglionic segment of HSCR mouse can generate functional neurons within the aganglionic gut environment and restore the neuromuscular activity of recipient mouse colon. We conclude that HSCR-derived SCs represent a potential autologous source of neural progenitor cells for regenerative therapy in HSCR.

Indexed as

Hirschsprung DiseaseNeural Stem CellsAnimalsMiceNeuronsSchwann Cellscell therapyenteric nervous systemHirschsprung diseaseSchwann cells

Identifiers

PMID36322091
PMCPMC9801298
OpenAlexW4307968322

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.