Evidence map›Paper›PMID 36321743›Full record

ArticleCartilage2022

Netrin-1 Secreted by Human Osteoarthritic Articular Chondrocytes Promotes Angiogenesis

Joulnar Akoum, Marie-Thérèse Corvol, Khadija Tahiri, Philippe Anract, David Biau, Didier Borderie, François Étienne, François Rannou, Christelle Nguyen

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cartilage, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Joulnar AkoumINSERM UMR-S 1124, Toxicité Environnementale, Cibles Thérapeutiques, Signalisation Cellulaire et Biomarqueurs (T3S), UFR Sciences Fondamentales et Biomédicales, Campus Saint-Germain-des-Prés, Université Paris Cité, Paris, France.
Marie-Thérèse CorvolINSERM UMR-S 1124, Toxicité Environnementale, Cibles Thérapeutiques, Signalisation Cellulaire et Biomarqueurs (T3S), UFR Sciences Fondamentales et Biomédicales, Campus Saint-Germain-des-Prés, Université Paris Cité, Paris, France.
Khadija TahiriINSERM UMR-S 1124, Toxicité Environnementale, Cibles Thérapeutiques, Signalisation Cellulaire et Biomarqueurs (T3S), UFR Sciences Fondamentales et Biomédicales, Campus Saint-Germain-des-Prés, Université Paris Cité, Paris, France.
Philippe AnractFaculté de Santé, UFR de Médecine, Université Paris Cité, Paris, France.
David BiauFaculté de Santé, UFR de Médecine, Université Paris Cité, Paris, France.
Didier BorderieINSERM UMR-S 1124, Toxicité Environnementale, Cibles Thérapeutiques, Signalisation Cellulaire et Biomarqueurs (T3S), UFR Sciences Fondamentales et Biomédicales, Campus Saint-Germain-des-Prés, Université Paris Cité, Paris, France.
François ÉtienneINSERM UMR-S 1124, Toxicité Environnementale, Cibles Thérapeutiques, Signalisation Cellulaire et Biomarqueurs (T3S), UFR Sciences Fondamentales et Biomédicales, Campus Saint-Germain-des-Prés, Université Paris Cité, Paris, France.
François RannouINSERM UMR-S 1124, Toxicité Environnementale, Cibles Thérapeutiques, Signalisation Cellulaire et Biomarqueurs (T3S), UFR Sciences Fondamentales et Biomédicales, Campus Saint-Germain-des-Prés, Université Paris Cité, Paris, France.
Christelle NguyenINSERM UMR-S 1124, Toxicité Environnementale, Cibles Thérapeutiques, Signalisation Cellulaire et Biomarqueurs (T3S), UFR Sciences Fondamentales et Biomédicales, Campus Saint-Germain-des-Prés, Université Paris Cité, Paris, France.ORCID 0000-0001-7141-3230
Inserm · FRUniversité Paris Cité · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveNetrin-1 expression in articular cartilage is correlated with osteoarthritic changes. We aimed to investigate the contribution of Netrin-1 secreted by human osteoarthritic articular chondrocytes to angiogenesis process

designHuman articular chondrocytes were extracted from non-osteoarthritic (

resultsAs compared with medium conditioned by non-osteoarthritic chondrocytes, medium conditioned by osteoarthritic chondrocytes permitted tube formation by HUVEC. Both non-osteoarthritic and osteoarthritic chondrocytes expressed Netrin-1 at the RNA and protein levels. At the RNA level, Netrin-1 expression did not differ between non-osteoarthritic and osteoarthritic chondrocytes. At the protein level, Netrin-1 appeared as a full protein of 64 kDa in non-osteoarthritic chondrocytes and as two cleaved proteins of 55 kDa and 64 kDa in osteoarthritic chondrocytes. Immunological neutralization of endogenous Netrin-1 reduced the pro-angiogenic and pro-inflammatory transcriptional profile of HUVEC treated with the medium conditioned by osteoarthritic chondrocytes, as well as their capacities to form tubes.

conclusionsMedium conditioned by osteoarthritic chondrocytes permits tube formation by HUVEC

Indexed as

Cartilage, ArticularEndothelial CellsChondrocytesHumansRNARNAangiogenesisarticular cartilagechondrocyteHUVECNetrin-1osteoarthritis

Identifiers

PMID36321743
PMCPMC9924986
OpenAlexW4307921350

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.