ArticleOrganic & biomolecular chemistry2022
Mutation of the eunicellane synthase Bnd4 alters its product profile and expands its prenylation ability.
Article in Organic & biomolecular chemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Sequence-guided engineering of a bacterial diterpene synthase induces further cyclization.ACS catalysis · 2026Article
- Biosynthetic formation of the 6/7/5-odyverdiene diterpenes via a 6/10-ACS catalysis · 2026Article
- Advances in semi-rational design of terpene synthases and their modifying enzymes.AMB Express · 2025Review
- Exploring and expanding the natural chemical space of bacterial diterpenes.Nature communications · 2025Article
- Canonical terpene synthases in arthropods: Intraphylum gene transfer.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Discovery of a terpene synthase synthesizing a nearly non-flexible eunicellane reveals the basis of flexibility.Nature communications · 2024Article
- Characterization of UbiA terpene synthases with a precursor overproduction system in Escherichia coli.Methods in enzymology · 2024Article
- Molecular insights into the catalytic promiscuity of a bacterial diterpene synthase.Nature communications · 2023Article
- FirstChem · 2023Article
- Biosynthesis, enzymology, and future of eunicellane diterpenoids.Journal of industrial microbiology & biotechnology · 2023Review
- Structure-guided product determination of the bacterial type II diterpene synthase Tpn2.Communications chemistry · 2022Article
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Authors and funding
4 authors.
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Abstract
Bnd4 catalyzes the first committed step in the biosynthesis of the bacterial diterpenoid benditerpenoic acid and was the first eunicellane synthase identified from nature. We investigated the catalytic roles of the aromatic residues in the active site of Bnd4 through a series of mutation studies. These experiments revealed that large hydrophobic or aromatic side chains are required at F162 and Y197 for eunicellane formation and that selected mutations at W316 converted Bnd4 into a cembrane synthase. In addition, the Bnd4
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