ArticleScientific reports2022
In vitro regeneration and its histological characteristics of Dioscorea nipponica Makino.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Advances in Research onMolecules (Basel, Switzerland) · 2026Review
- Nanomaterial-induced modulation of morpho-physio-biochemical attributes and enhancement of anticancer compounds in Tagetes erecta L. callus cultures.Scientific reports · 2026Article
- Mild Drought Promotes Biomass Accumulation and Increases Diosgenin Content in Rhizomes ofPlants (Basel, Switzerland) · 2025Article
- Key Techniques in Tissue Culture of Scape Explants fromPlants (Basel, Switzerland) · 2025Article
- Efficient in vitro regeneration and overcoming premature senescence of Chenopodium quinoa willd.Scientific reports · 2025Article
- Isolation of endophytes from Dioscorea nipponica Makino for stimulating diosgenin production and plant growth.Plant cell reports · 2024Article
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Authors and funding
4 authors.
Funding
Abstract
Dioscorea nipponica Makino is an optimal candidate to develop the diosgenin industry in North China. Due to its increasing demand in the medicine industry, it is urgent to apply new biotechnological tools to foster breeds with desirable traits and enhanced secondary metabolite production. The production of useful metabolites by the in vitro cultured rhizomes can be explored successfully for utilization by various food and drug industries. In this study, we reported callus formation and plantlet regeneration of the medicinal plant D. nipponica. Explants of leaves, stem segments and rhizomes of aseptic seedlings were cultured on Murashige and Skoog (MS) medium containing various combinations of auxin and cytokinin to find the optimal PGRs of each type of explant for callus induction and shoot regeneration of D. nipponica. The paraffin section technique was also used to observe of the morphogenesis of callus and adventitious bud. Explants of seeds and rhizomes formed calli at high frequency in all lines we examined. However, the explant of leaves rarely formed callus. Three kinds of callus were detected during the induction phase. Here, we describe three types of callus (Callus I-III) with different structure characteristics. Greenish in color and a nodule-like protrusion surface (Callus type III) were arranged more closely of cells with less interstitial substance, cell differentiation ability stronger than other callus types. The optimum combination was the maximum shoot differentiation frequency of 90% in callus derived from seeds cultured on MS medium with 2.0 mg L
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