Evidence map›Paper›PMID 36319799›Full record

ArticleCell regeneration (London, England)2022

Mechanical stretching boosts expansion and regeneration of intestinal organoids through fueling stem cell self-renewal.

Fanlu Meng, Congcong Shen, Li Yang, Chao Ni, Jianyong Huang, Kaijun Lin, Zanxia Cao, Shicai Xu, Wanling Cui, Xiaoxin Wang and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell regeneration (London, England), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Article
  5. Review
  6. Article
  7. Article
  8. Organoids for disease modeling and treatment: state-of-the-art.Experimental hematology & oncology · 2026
    Review
  9. Review
  10. Article
  11. Article
  12. Review
  13. Article
  14. Review
  15. Article
  16. Review
  17. Article
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  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Fanlu Meng *Shandong Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou, 253023, China.
Congcong Shen *Shandong Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou, 253023, China.
Li YangState Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Fudan University, Shanghai, 200438, China.
Chao NiState Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Fudan University, Shanghai, 200438, China.
Jianyong HuangDepartment of Mechanics and Engineering Science, College of Engineering, Peking University, Beijing, 100871, China.
Kaijun LinState Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Fudan University, Shanghai, 200438, China.
Zanxia CaoShandong Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou, 253023, China.
Shicai XuShandong Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou, 253023, China.
Wanling CuiShandong Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou, 253023, China.
Xiaoxin WangShandong Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou, 253023, China.
Bailing ZhouShandong Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou, 253023, China.
Chunyang XiongDepartment of Mechanics and Engineering Science, College of Engineering, Peking University, Beijing, 100871, China. cyxiong@pku.edu.cn.ORCID https://orcid.org/0000-0002-8821-2643
Jihua WangShandong Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou, 253023, China. jhw25336@126.com.
Bing ZhaoState Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Fudan University, Shanghai, 200438, China. bingzhao@fudan.edu.cn.ORCID https://orcid.org/0000-0001-9891-3569

Funding

National Key Research and Development Program of China Stem Cell and Translational Research 2018YFA0109400National Natural Science Foundation of China 11972001National Natural Science Foundation of China 11972002National Natural Science Foundation of China 32022022National Natural Science Foundation of China 62071085Natural Science Foundation of Beijing Municipality Z200017Natural Science Foundation of Shandong Province ZR2019PC026Open Research Fund of Shandong Key Laboratory of Biophysics of Dezhou College SD2018BP005Shanghai Pilot Program for Basic Research - Fudan University 21TQ1400100 21TQ003
6 · The paper itself

Abstract

Intestinal organoids, derived from intestinal stem cell self-organization, recapitulate the tissue structures and behaviors of the intestinal epithelium, which hold great potential for the study of developmental biology, disease modeling, and regenerative medicine. The intestinal epithelium is exposed to dynamic mechanical forces which exert profound effects on gut development. However, the conventional intestinal organoid culture system neglects the key role of mechanical microenvironments but relies solely on biological factors. Here, we show that adding cyclic stretch to intestinal organoid cultures remarkably up-regulates the signature gene expression and proliferation of intestinal stem cells. Furthermore, mechanical stretching stimulates the expansion of SOX9

Indexed as

Intestinal organoidLgr5+ stem cellMechanical stretchingRegenerationWnt/β-catenin signaling

Identifiers

PMID36319799
PMCPMC9626719

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.